Translational Landscape of Photomorphogenic Arabidopsis
Translational Landscape of Photomorphogenic Arabidopsis
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DOI：
10.1105/tpc.113.114769
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发表时间：
2013-10-01
期刊：
影响因子：
11.6
通讯作者：
Wu, Shu-Hsing
中科院分区：
文献类型：
--
作者：
Liu, Ming-Jung;Wu, Szu-Hsien;Wu, Shu-Hsing
翻译调控在基因表达调控中起着至关重要的作用。为了破译光形态发生拟南芥翻译调控的分子基础，我们采用核糖体图谱的方法，在黑暗和光照下定位了拟南芥黄化幼苗中翻译核糖体的全基因组位置。我们发现，在拟南芥中，翻译的核糖体保护一个类似于30个核苷酸的区域，并以三个核苷酸的周期移动，这一特征也在酿酒酵母和哺乳动物中观察到。光促进了参与叶绿体组织和功能的基因的翻译。上游开放阅读框架是由ATG启动的，而不是CTG介导的下游主要开放阅读框架的翻译抑制。此外，我们还观察到在光照和黑暗生长的拟南芥幼苗中广泛存在的microRNA靶基因的翻译抑制。这种在核苷酸解析水平上进行翻译的转录本的全基因组特征表明，多种翻译机制的组合协调和微调了具有环境响应性的植物中不同转录本的翻译。
Translational control plays a vital role in regulating gene expression. To decipher the molecular basis of translational regulation in photomorphogenic Arabidopsis thaliana, we adopted a ribosome profiling method to map the genome-wide positions of translating ribosomes in Arabidopsis etiolated seedlings in the dark and after light exposure. We found that, in Arabidopsis, a translating ribosome protects an similar to 30-nucleotide region and moves in three-nucleotide periodicity, characteristics also observed in Saccharomyces cerevisiae and mammals. Light enhanced the translation of genes involved in the organization and function of chloroplasts. Upstream open reading frames initiated by ATG but not CTG mediated translational repression of the downstream main open reading frame. Also, we observed widespread translational repression of microRNA target genes in both light- and dark-grown Arabidopsis seedlings. This genome-wide characterization of transcripts undergoing translation at the nucleotide-resolution level reveals that a combination of multiple translational mechanisms orchestrates and fine-tunes the translation of diverse transcripts in plants with environmental responsiveness.