THE DISTRIBUTION OF HISTIDINE-DECARBOXYLASE MESSENGER-RNA IN THE RAT-BRAIN - AN INSITU HYBRIDIZATION STUDY USING SYNTHETIC OLIGONUCLEOTIDE PROBES
THE DISTRIBUTION OF HISTIDINE-DECARBOXYLASE MESSENGER-RNA IN THE RAT-BRAIN - AN INSITU HYBRIDIZATION STUDY USING SYNTHETIC OLIGONUCLEOTIDE PROBES
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DOI：
10.1016/0304-3940(90)90181-8
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发表时间：
1990-11-27
影响因子：
2.5
通讯作者：
PANULA, P
中科院分区：
文献类型：
--
作者：
CASTREN, E;PANULA, P
L-组氨酸脱羧酶催化L-组氨酸形成组胺。用人工合成的寡核苷酸探针进行原位杂交，研究了成年大鼠脑内表达组氨酸脱羧酶基因的神经元的分布。在下丘脑结节乳头核中检测到组氨酸脱羧酶mRNA的表达，该核团具有组氨酸脱羧酶和组胺样免疫反应，但在其他脑区未见表达。这种方法可能有助于研究中枢组胺能神经元的生理作用。
L-Histidine decarboxylase catalyzes the formation of histamine from the amino acid L-histidine. We have studied the distribution of neurons expressing mRNA for histidine decarboxylase in adult rat brain using in situ hybridization with synthetic oligonucleotide probes. The expression of mRNA for histidine decarboxylase was detected in the hypothalamic tuberomammillary nucleus that has been shown to contain histidine decarboxylase-like and histamine-like immunoreactivity, but not in any other brain area. This method may prove useful in studying the physiological role of central histaminergic neurons.