Enzyme linked immunosorbent assay for total potent estrogenic miroestrol and deoxymiroestrol of Pueraria candollei, a Thai herb for menopause remedy
Enzyme linked immunosorbent assay for total potent estrogenic miroestrol and deoxymiroestrol of Pueraria candollei, a Thai herb for menopause remedy
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酶联免疫吸附测定葛根（一种用于治疗更年期的泰国草药）的总有效雌激素微雌酚和脱氧微雌酚
DOI：
10.1007/s11418-018-1194-x
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发表时间：
2018
影响因子：
3.3
通讯作者：
W. Putalun
中科院分区：
文献类型：
--
作者：
G. Yusakul;T. Kitisripanya;T. Juengwatanatrakul;S. Sakamoto;Hiroyuki Tanaka;W. Putalun
Miroestrol and deoxymiroestrol are the most potent phytoestrogens ofPueraria candolleivar.mirifica, having been proved as an effective herb for menopausal symptoms in folk medicines and clinical trials. To ensure efficacy and safety ofP. candolleivar.mirificainvolved in nutraceutical products being available worldwide, the content of potent phytoestrogens as active ingredients should be specified. Therefore, in this study, we produced a monoclonal antibody for total analysis of potent estrogenic miroestrol and deoxymiroestrol, for which an analytical method was developed using a procedure for an indirect competitive enzyme-linked immunosorbent assay. The antibody exhibited equal reactivity against miroestrol and deoxymiroestrol. The sensitivity of determination was in the range of 31.3–500 ng/ml with high precision. The analytical parameters, such as accuracy (99.6–106% recovery) and high correlation with a HPLC–UV method, indicated the reliability of analysis. This method is of high performance, and it is cheap to control optimal miroestrol and deoxymiroestrol doses ofP. candolleivar.mirificanutraceutical products.
影响因子：
4.9
作者：
Urasopon, Nontakorn;Hamada, Yuzuru;Malaivijitnond, Suchinda
通讯作者：
Malaivijitnond, Suchinda