The matrix metalloproteinase-14 (MMP-14) gene is structurally distinct from other MMP genes and is co-expressed with the TIMP-2 gene during mouse embryogenesis
The matrix metalloproteinase-14 (MMP-14) gene is structurally distinct from other MMP genes and is co-expressed with the TIMP-2 gene during mouse embryogenesis
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DOI：
10.1074/jbc.272.41.25511
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发表时间：
1997-10-10
影响因子：
4.8
通讯作者：
Olsen, BR
中科院分区：
文献类型：
--
作者：
Apte, SS;Fukai, N;Olsen, BR
基质金属蛋白酶（matrix metalloproteinases，MMPs）是一类含锌的基质降解内肽酶。最近已经描述了膜型（MT）-MMPs的亚家族。我们已经确定了编码第一个MT-MMP的基因（Mmp 14）的结构，MT 1-MMP（MMP-14），并将其定位到小鼠染色体14。小鼠MMP-14蛋白由10个外显子编码。MMP-14的新的C-末端肽结构域由单个大外显子编码，该外显子还编码3 '-非翻译区。编码MMP-14的催化结构域和前结构域的外显子的结构不同于先前描述的MMP基因，而编码血红素蛋白样结构域的外显子与大多数其他MMP基因的外显子相似。Mmp 14和金属蛋白酶组织抑制剂2（TIMP 2）的基因在小鼠发育过程中表现出时间和空间上的共调节表达。它们在血管和泌尿生殖系统发育期间以及骨软骨和肌肉腱结构发育期间共表达。这两个基因的严格共表达表明共同的调节途径，可能具有重要的功能意义的明胶酶A前体的激活在健康和疾病。
The matrix metalloproteinases (MMPs) are a family of zinc-containing matrix degrading endopeptidases. A subfamily of membrane type (MT) -MMPs has been described recently. We have determined the structure of the gene (Mmp14) encoding the first MT-MMP to be described, MT1-MMP (MMP-14), and mapped it to mouse chromosome 14. The mouse MMP-14 protein is encoded by ten exons. The novel C-terminal peptide domains of MMP-14 are encoded by a single large exon that also encodes the 3'-untranslated region, The structure of the exons encoding the catalytic domain and pro domain of MMP-14 is distinct from previously described MMP genes, whereas the exons encoding the hemopexin-like domains are similar to those of most other MMP genes. Mmp14 and the gene for tissue inhibitor of metalloproteinases-2 (Timp2) show a temporally and spatially coregulated expression during mouse development. They are co-expressed during vascular and urogenital development and during the development of osteocartilaginous and musculotendinous structures. The stringent co-expression of these two genes suggests common regulatory pathways that may have important functional implications for the activation of pro-gelatinase A in health and disease.