Brain-derived Neurotrophic Factor Promotes the Migration of Olfactory Ensheathing Cells Through TRPC Channels
Brain-derived Neurotrophic Factor Promotes the Migration of Olfactory Ensheathing Cells Through TRPC Channels
复制标题
脑源性神经营养因子通过 TRPC 通道促进嗅鞘细胞迁移
DOI：
10.1002/glia.23049
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发表时间：
2016-12-01
期刊：
影响因子：
6.2
通讯作者：
Huang, Zhi-Hui
中科院分区：
文献类型：
--
作者：
Wang, Ying;Teng, Hong-Lin;Huang, Zhi-Hui
嗅鞘细胞（Olfactory enshe鞘细胞，OECs）是一类独特的神经胶质细胞，具有促进嗅觉系统轴突生长的特性。oec有组织的迁移对神经再生和嗅觉发育至关重要。然而，OEC迁移的分子机制尚不清楚。在本研究中，我们研究了脑源性神经营养因子（BDNF）对OEC迁移的影响。最初，“划痕”迁移实验、倒盖和Boyden室迁移实验表明，BDNF可以促进原代培养oec的迁移。此外，在单细胞迁移实验中，BDNF梯度吸引了oec的迁移。机制上，在OEC中表达的TrkB受体介导BDNF诱导的OEC迁移，BDNF触发OEC中的钙信号。最后，在OEC中高表达的瞬时受体电位阳离子通道（TRPCs）负责bdnf诱导的钙信号，并且是bdnf诱导的OEC迁移所必需的。综上所述，这些结果表明BDNF促进了培养的OEC的迁移，一个意想不到的发现是trpc是BDNF诱导的OEC迁移所必需的。
Olfactory ensheathing cells (OECs) are a unique type of glial cells with axonal growth-promoting properties in the olfactory system. Organized migration of OECs is essential for neural regeneration and olfactory development. However, the molecular mechanism of OEC migration remains unclear. In the present study, we examined the effects of brain-derived neurotrophic factor (BDNF) on OEC migration. Initially, the "scratch" migration assay, the inverted coverslip and Boyden chamber migration assays showed that BDNF could promote the migration of primary cultured OECs. Furthermore, BDNF gradient attracted the migration of OECs in single-cell migration assays. Mechanistically, TrkB receptor expressed in OECs mediated BDNF-induced OEC migration, and BDNF triggered calcium signals in OECs. Finally, transient receptor potential cation channels (TRPCs) highly expressed in OECs were responsible for BDNF-induced calcium signals, and required for BDNF-induced OEC migration. Taken together, these results demonstrate that BDNF promotes the migration of cultured OECs and an unexpected finding is that TRPCs are required for BDNF-induced OEC migration.