High-Throughput Screening of Antimicrobial Resistance Genes and Their Association With Class 1 Integrons in Urban Rivers in Japan
High-Throughput Screening of Antimicrobial Resistance Genes and Their Association With Class 1 Integrons in Urban Rivers in Japan
复制标题
DOI：
10.3389/fenvs.2022.825372
复制
发表时间：
2022-02-04
影响因子：
4.6
通讯作者：
Furumai, Hiroaki
中科院分区：
文献类型：
--
作者：
Kasuga, Ikuro;Nagasawa, Kyoka;Furumai, Hiroaki
抗生素耐药性(AMR)是一个严重的公共卫生问题。许多国家已经实施了对人和动物的AMR监测计划，但环境中AMR的监测计划尚未建立。1类整合子可以获得对基因盒的抗菌素耐药基因(ARGs)，被认为是评估人类对AMR影响的候选基因。然而，水环境中1类整合子与ARGs之间的联系研究较少，需要进一步阐明。本研究采用高通量定量聚合酶链式反应(HT-qPCR)技术，对东京及周边地区24条城市河流中ARGs和移动基因元件(MGES)的污染特征进行了研究。还测定了1类整合子整合酶基因(IntI1)的丰度和1类整合子基因盒的排列。总体而言，每个样本检测到9-53个靶基因，并且它们的丰度随着污水处理厂的排放而增加。根据河流和废水的HT-qPCR谱对其进行了分类，表明该方法可用于表征水环境的污染状况。观察河水中INTI1的流行情况。部分ARGs和MGES与intI1呈正相关，表明intI1可以作为监测城市河流中这些ARGs和MGES的指标。1类整合子基因盒的长读测序显示，基因盒中存在一到三个ARG。无论样本类型如何，在基因盒中均以bla(GES-24)、aadA2和QacH占优势。应进一步监测携带这些args的1类整合子在水环境中的来源和传播。
Antimicrobial resistance (AMR) is a serious public health concern. Many countries have implemented AMR surveillance programs for humans and animals, but a scheme for monitoring AMR in the environment has not yet been established. Class 1 integrons, which can acquire antimicrobial resistance genes (ARGs) to gene cassettes, were proposed as a candidate to evaluate the anthropogenic impacts on AMR. However, the association between class 1 integrons and ARGs in aquatic environments is less studied and requires further elucidation. This study used high-throughput quantitative polymerase chain reaction (HT-qPCR) to characterize the pollution profiles of ARGs and mobile gene elements (MGEs) in 24 urban rivers in Tokyo and its surrounding area. The abundance of class 1 integron-integrase gene (intI1) and the array of class 1 integron gene cassettes were also determined. In total, 9-53 target genes were detected per sample, and their abundances increased following effluent discharge from wastewater treatment plants. The river and wastewater samples were categorized based on their HT-qPCR profiles, indicating that this method was useful for characterizing the pollution status in aquatic environments. The prevalence of intI1 in the rivers was observed. Some ARGs and MGEs were positively correlated with intI1, indicating that intI1 could be used as a proxy for monitoring these ARGs and MGEs in urban rivers. Long-read sequencing of class 1 integron gene cassettes revealed that one to three ARGs were present in the gene cassettes. Regardless of the sample type, bla(GES-24), aadA2, and qacH were dominant in the gene cassettes. The source and spread of class 1 integrons carrying these ARGs in aquatic environments should be further monitored.