Exploration of the Substrate Diversity of Leucoanthocyanidin Reductases
Exploration of the Substrate Diversity of Leucoanthocyanidin Reductases
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无色花青素还原酶底物多样性的探索
DOI：
10.1021/acs.jafc.9b06353
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发表时间：
2020
影响因子：
6.1
通讯作者：
Xia Tao
中科院分区：
文献类型：
--
作者：
Zhang Lingjie;Wang Peiqiang;Ma Xue;Zhao Wenyan;Li Ming;Yao Shengbo;Liu Yajun;Gao Liping;Xia Tao
原花青素（PA）主要由表儿茶素（EC）或儿茶素（C）亚基组成。 C型儿茶素（C和GC）通常被认为是由无色花青素还原酶（LAR）催化的。在本研究中，我们重新评估了LAR的功能。LcLAR1是从富含C型儿茶素的百脉中分离得到的。烟草中LcLAR1的过度表达导致EC和EC-葡萄糖苷含量显着增加。拟南芥中 LcLAR1 的过表达促进了可溶性 PA 的积累，包括 EC、PA 二聚体和 PA 三聚体。然而，在过表达LcLAR1的转基因突变体中，C和C-葡萄糖苷的含量增加。此外，LcLAR1 过表达。百脉根导致C水平显着增加。综上所述，LcLAR1的产物依赖于底物，这揭示了LcLAR1的底物多样性。我们的研究为类黄酮途径，特别是 LAR 的作用提供了新的见解。
Proanthocyanidins (PAs) are mainly composed of epicatechin (EC) or catechin (C) subunits. C-type catechins (C and GC) are generally considered to be catalyzed by leucocyanidin reductase (LAR). In this study, we re-evaluated the function of LAR.LcLAR1was isolated fromLotus corniculatus, which is rich in C-type catechins. Overexpression ofLcLAR1in tobacco resulted in a significantly increased content of EC and EC-glucoside. Overexpression ofLcLAR1inArabidopsis thalianapromoted the accumulation of soluble PAs, including EC, PA dimers, and PA trimers. However, in the transgenicansmutant overexpressingLcLAR1, the contents of C and C-glucoside were increased. In addition, overexpression ofLcLAR1inL. corniculatusresulted in a significant increase of C levels. Taken together, the products ofLcLAR1 depended on the substrates, which revealed the substrate diversity ofLcLAR1. Our study provides new insights into the flavonoid pathway, especially the role of LAR.