Biochip for the Detection of Bacillus anthracis Lethal Factor and Therapeutic Agents against Anthrax Toxins.
Biochip for the Detection of Bacillus anthracis Lethal Factor and Therapeutic Agents against Anthrax Toxins.
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DOI：
10.3390/membranes6030036
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发表时间：
2016-06-24
期刊：
影响因子：
4.2
通讯作者：
Robertson JW
中科院分区：
文献类型：
--
作者：
Silin V;Kasianowicz JJ;Michelman-Ribeiro A;Panchal RG;Bavari S;Robertson JW
栓系脂质双分子层膜（tBLMs）在生物传感和膜蛋白结构研究等领域有着广泛的应用。本报告描述了一种炭疽毒素生物传感器，该传感器是通过tBLM与炭疽杆菌保护性抗原离子通道的自组装制备的，该离子通道既是识别元件又是电化学传感器。利用电化学阻抗谱和表面等离子体共振对传感器及其性能进行了表征。该传感器显示出与ELISA类似的灵敏度，也可用于快速筛选与毒素结合并可能抑制其致命作用的分子。
Tethered lipid bilayer membranes (tBLMs) have been used in many applications, including biosensing and membrane protein structure studies. This report describes a biosensor for anthrax toxins that was fabricated through the self-assembly of a tBLM with B. anthracis protective antigen ion channels that are both the recognition element and electrochemical transducer. We characterize the sensor and its properties with electrochemical impedance spectroscopy and surface plasmon resonance. The sensor shows a sensitivity similar to ELISA and can also be used to rapidly screen for molecules that bind to the toxins and potentially inhibit their lethal effects.