ENZYME-LINKED-IMMUNOSORBENT-ASSAY FOR PARAQUAT AND ITS APPLICATION TO EXPOSURE ANALYSIS
ENZYME-LINKED-IMMUNOSORBENT-ASSAY FOR PARAQUAT AND ITS APPLICATION TO EXPOSURE ANALYSIS
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DOI：
10.1021/ac00121a057
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发表时间：
1986-07-01
影响因子：
7.4
通讯作者：
SEIBER, JN
中科院分区：
文献类型：
--
作者：
VANEMON, J;HAMMOCK, B;SEIBER, JN
建立了人体暴露样品中百草枯的酶联免疫吸附测定方法。使用1/5000的抗体稀释度，可测量0.1-27 ng/mL范围内的百草枯阳离子浓度。根据所分析的基质不同，检出限在0.1~1.0 ng/mL之间。该方法精密度好，批间精密度小于5%，批间精密度小于4%，对百草枯有较好的选择性，与百草枯、四氟草胺等化合物的交叉反应最小。与气相色谱方法相比，该方法具有回收率高、劳动强度小、灵敏度高等优点。酶联免疫吸附试验被应用于在空中喷洒棉花期间收集的大容量玻璃纤维过滤器、个人空气监测器、工人衣服贴片和洗手液中的百草枯。当同一滤器同时用ELISA法和GC法分析时，ELISA法的样品制备步骤的效率更高，其结果一致。由于其选择性、灵敏度和简单易行，免疫分析法可用于测定多种化合物(1)。以往关于开发农药特异性抗体的报道表明，这种免疫化学方法比气相色谱(GC)和高效液相色谱(HPLC)方法具有优势(1，2)。例如，对S生物丙烯菊酯(3，4)、对硫磷(5)和其他农药(6-11)的免疫检测证明了免疫化学技术在环境领域的适用性。对于水溶性化合物或低挥发性化合物，免疫分析方法可以比现在使用的分析方法更快、更便宜、更灵敏和更具重复性。
An enzyme-linked Immunosorbent assay (ELISA) was developed for the determination of paraquat In humanexposure samples. Using an antibody dilution of 1/5000, concentrations of paraquat cation In the range 0.1-27 ng/mL could be measured. Limit of detection ranged from 0.1 to 1.0 ng/mL depending on the matrix analyzed. The method had good precision, with less than 5% between-run and less than 4% wlthln-run variation, and was selective for paraquat showing minimal cross reactivity with ethylparaquat, cflquat, and other compounds. In comparison with a gas chromatographic method, the ELISA gave higher recoveries, was less labor Intensive, and was more sensitive. The ELISA was applied to paraquat In high volume glassfiber filters, personal air monitors, worker clothing patches, and hand washes collected during aerial spraying of cotton. When the same filters were analyzed by both ELISA and GC, the ELISA consistently resulted In higher values In keeping with the greater efficiency of the sample preparation steps of ELISA.Due to their selectivity, sensitivity, and simplicity, immu-noassays are useful for determining a wide variety of com-pounds (1). Previous reports on the development of pesticide-specific antibodies indicate such immunochemical pro-cedures can offer advantages over gas chromatography (GC) and high-performance liquid chromatographic (HPLC) methods (1, 2). For instance, immunoassays for S-bioallethrin (3, 4), parathion (5), and other pesticides (6-11) demonstrate the applicability of immunochemicaltechnology in environmental areas. For water-soluble compounds or compounds with low volatility, immunoassays can be faster, cheaper, and significantly more sensitive and reproducible than analytical procedures now employed.