β-Glucan Extracted from Highland Barley Alleviates Dextran Sulfate Sodium-Induced Ulcerative Colitis in C57BL/6J Mice.
β-Glucan Extracted from Highland Barley Alleviates Dextran Sulfate Sodium-Induced Ulcerative Colitis in C57BL/6J Mice.
复制标题
青稞提取的β-葡聚糖可减轻硫酸葡聚糖钠诱导的 C57BL/6J 小鼠溃疡性结肠炎
DOI：
10.3390/molecules26195812
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发表时间：
2021-09-25
期刊：
影响因子：
--
通讯作者：
Li X
中科院分区：
文献类型：
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作者：
Chen M;Tian S;Li S;Pang X;Sun J;Zhu X;Lv F;Lu Z;Li X
炎症性肠病（IBD）对人类健康有重大影响，主要有两种表现形式：克罗恩病和溃疡性结肠炎（UC）。青稞是藏族最常见的粮食作物，其β - 葡聚糖含量比其他任何作物都高得多。青稞β - 葡聚糖（HBBG）能够缓解胃肠功能紊乱，促进肠道健康。本研究旨在评估HBBG是否能够缓解小鼠的溃疡性结肠炎。通过在饮用水中添加2%葡聚糖硫酸钠（DSS）持续1周建立小鼠溃疡性结肠炎模型。引入HBBG饮食后，溃疡性结肠炎得到缓解，表现为疾病活动指数（DAI）评分降低、组织病理学损伤减轻、结肠髓过氧化物酶（MPO）浓度降低以及结肠萎缩情况改善。此外，我们发现HBBG能够提高编码紧密连接蛋白ZO - 1、克劳丁 - 1、闭合蛋白以及黏蛋白2（MUC2）的基因的相对转录水平，从而降低肠道通透性。另外，HBBG维持了促炎细胞因子和抗炎细胞因子的平衡，并调节了肠道菌群的结构。
Inflammatory bowel disease (IBD), which significantly affects human health, has two primary presentations: Crohn’s disease and ulcerative colitis (UC). Highland barley is the most common food crop for Tibetans and contains much more β-glucan than any other crop. Highland barley β-glucan (HBBG) can relieve the gastrointestinal dysfunction and promote intestines health. This study aimed to evaluate whether HBBG can relieve UC in mice. A mouse model of UC was established by adding 2% dextran sulfate sodium (DSS) to drinking water for 1 week. UC was alleviated after the introduction of the HBBG diet, as indicated by reductions in the disease activity index (DAI) score, histopathological damage, and the concentration of colonic myeloperoxidase (MPO), along with an improvement in colonic atrophy. Furthermore, we found that HBBG can increase the relative transcriptional levels of genes encoding ZO-1, claudin-1, occludin, and mucin2 (MUC2), thereby reducing intestinal permeability. Additionally, HBBG maintained the balance of proinflammatory and anti-inflammatory cytokines and modulated the structure of the intestinal flora.