RADIOIMMUNOASSAY OF THE ATTACK COMPLEX OF COMPLEMENT IN SERUM FROM PATIENTS WITH SYSTEMIC LUPUS-ERYTHEMATOSUS
RADIOIMMUNOASSAY OF THE ATTACK COMPLEX OF COMPLEMENT IN SERUM FROM PATIENTS WITH SYSTEMIC LUPUS-ERYTHEMATOSUS
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DOI：
10.1056/nejm198506203122502
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发表时间：
1985-01-01
影响因子：
158.5
通讯作者：
MICHAEL, A
中科院分区：
文献类型：
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作者：
FALK, RJ;DALMASSO, AP;MICHAEL, A
本文应用放射免疫法测定了系统性红斑狼疮患者血清补体攻击复合物（SC 5 b-9）。放射免疫测定法使用针对C9抗原的单克隆抗体，C9抗原在天然C9中不存在，但存在于SC 5 b-9上。在63名正常受试者中有13名可检测到SC 5 b-9，平均值为0.5单位（范围为0至4.8），在14名活动性狼疮患者中有13名升高。对108例狼疮患者样本的分析显示，当疾病活动时，平均值为10.1单位（范围，0至35），当疾病临床稳定时，平均值为1.0单位（范围，0至4.3）。SC 5 b-9是比C3、C4或CH 50更敏感的疾病活动性指标;其特异性与C3和C4相当。49例其他类型肾小球肾炎患者中有6例血清中SC 5 b-9升高，我们的研究记录了活动性系统性红斑狼疮患者血清中SC 5 b-9的异常，并将其升高与临床疾病活动性相关。（N Engl J Med 1985; 312：1594-9.）
We developed a radioimmunoassay to measure the attack complex of complement (SC5b–9) in serum from patients with systemic lupus erythematosus. The radioimmunoassay used a monoclonal antibody to an antigen of C9, which is absent from native C9 but present on SC5b–9. SC5b–9 was detectable in 13 of 63 normal subjects, with a mean value of 0.5 unit (range, 0 to 4.8), and elevated in 13 of 14 patients with active lupus. Analysis of 108 samples from the patients with lupus revealed a mean value of 10.1 units (range, 0 to 35) when the disease was active and 1.0 unit (range, 0 to 4.3) when it was clinically stable. SC5b–9 was a more sensitive measure of disease activity than C3, C4, or CH50; its specificity was equivalent to that of C3 and C4. SC5b–9 was elevated in serum from 6 of 49 patients with other forms of glomerulonephritis.Our study documents the presence of SC5b–9 in abnormal serum and correlates its elevation with clinical disease activity in patients with active systemic lupus erythematosus. (N Engl J Med 1985; 312:1594–9.)