The Feat of Packaging Eight Unique Genome Segments.
The Feat of Packaging Eight Unique Genome Segments.
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DOI：
10.3390/v8060165
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发表时间：
2016-06-17
期刊：
影响因子：
--
通讯作者：
Schwemmle M
中科院分区：
文献类型：
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作者：
Giese S;Bolte H;Schwemmle M
甲型流感病毒(IAV)含有一个分段的RNA基因组，该基因组被组织成八个不同的病毒核糖核蛋白(VRNP)复合体。尽管片段基因组可能是适应新宿主环境的主要优势，但它是以高度复杂的基因组包装机制为代价的。新合成的vRNPs克服了细胞内体循环机制，进入了病毒在质膜上的萌发部位。每个RNA基因组片段特有的基因组包装序列被认为是确保八个不同的vRNPs组装和整合到后代病毒颗粒中的关键决定因素。最近使用先进的荧光显微镜技术的研究表明，vRNP亚束(由不到8个vRNP组成)在它们通过循环内小体运输的过程中形成。这种亚束的形成可能是在萌发地点有效包装八个不同基因组片段所必需的，这进一步突出了IAV基因组包装的复杂性。
Influenza A viruses (IAVs) harbor a segmented RNA genome that is organized into eight distinct viral ribonucleoprotein (vRNP) complexes. Although a segmented genome may be a major advantage to adapt to new host environments, it comes at the cost of a highly sophisticated genome packaging mechanism. Newly synthesized vRNPs conquer the cellular endosomal recycling machinery to access the viral budding site at the plasma membrane. Genome packaging sequences unique to each RNA genome segment are thought to be key determinants ensuring the assembly and incorporation of eight distinct vRNPs into progeny viral particles. Recent studies using advanced fluorescence microscopy techniques suggest the formation of vRNP sub-bundles (comprising less than eight vRNPs) during their transport on recycling endosomes. The formation of such sub-bundles might be required for efficient packaging of a bundle of eight different genomes segments at the budding site, further highlighting the complexity of IAV genome packaging.