Isolation of pregn-4-ene-17α, 20β-diol-3-one from the plasma of pacific salmon (oncorhynchus,nerka)
Isolation of pregn-4-ene-17α, 20β-diol-3-one from the plasma of pacific salmon (oncorhynchus,nerka)
复制标题
从太平洋鲑鱼 (oncorhynchus,nerka) 血浆中分离 pregn-4-ene-17α, 20β-diol-3-one
DOI：
10.1016/0006-291x(60)90204-7
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发表时间：
1960
影响因子：
3.1
通讯作者：
A. Ronald
中科院分区：
文献类型：
--
作者：
D. Idler;U. Fagerlund;A. Ronald
材料和方法1959年9月在不列颠哥伦比亚省奇尔科湖采集了约80条产卵后的雌性红鲑鱼，仅通过切断尾动脉流血的。采取预防措施以排除血液中的粘液和排泄物。从肝素化血液制备血浆（1640 ml），并在干冰上冷冻。解冻的血浆用2.5倍体积的CH2Cl2 MS提取3次。冷却的提取物用O. OsN NaOR，O.在30 ° C下除去IN乙酸和Li2O和Li2Cl2。在真空中。将残余物溶于70%甲醇中，用己烷分配除去非甾体脂肪物质。将色谱纸预干燥（Idler &al.，1959年）。溶剂133
Materials and Uethods Approximately 80 post-spawned female sockeye salmon, taken at Chilko Lake, BC in September, 1959, mere bled by severing the caudal artery. Precautions were taken to exclude slime and excreta from the blood. Plasma (1640 ml) was prepared from the heparinized blood and frozen on dry ice. The thawed plasma MS extracted 3 times with 2.5 volumes of CH2C12. The chilled extract was washed with O. OsN NaOR, O. lN acetic acid and If20 and the Ui2C12 removed at 30. in vacua. The residue was taken up in 70% methanol and nonsteroid fatty material removed by partition with hexane. Chromatography paper was pre-uashed (Idler &al., 1959). Solvent 133