Detection of biofilm-forming strains of Staphylococcus epidermidis and S. aureus.
Detection of biofilm-forming strains of Staphylococcus epidermidis and S. aureus.
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DOI：
10.1586/14737159.2.5.478
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发表时间：
2002-09-01
影响因子：
5.1
通讯作者：
Montanaro, Lucio
中科院分区：
文献类型：
--
作者：
Arciola, Carla Renata;Campoccia, Davide;Montanaro, Lucio
生物被膜的形成是表皮葡萄球菌和葡萄球菌的重要毒力因子。金黄色。细菌聚集形成生物膜的能力与产生通常称为粘液的细胞外粘液物质的能力严格相关，粘液物质的主要成分具有多糖性质并由糖胺聚糖组成。近年来，基于PCR的新分子技术与更传统的方法一起用于鉴定毒性生物膜形成菌株。控制这种胞外多糖产生的基因，特别是icaA、icaC和icaD基因的检测，为我们提供了一种快速准确的菌株表征技术。然而，仍然需要完善的方法，如刚果红琼脂试验，以确认可能的相变异菌株的表型表达。在未来，表型调制的遗传机制的完整知识，理解所有的调控基因，可以允许的特点，只是通过分子手段在一个单一的步骤分离。
The formation of biofilm represents an important virulence factor of certain strains of Staphylococcus epidermidis and S. aureus. The ability of bacteria to aggregate, forming biofilms, is strictly related to the capacity of producing an extracellular mucoid substance often referred to as slime, whose main component is of polysaccharidic nature and consists of glycosaminoglycans. In recent years, new molecular techniques based on PCR have come alongside more traditional methods for identification of virulent biofilm-forming strains. The detection of the genes governing the production of such extracellular polysaccharide and, in particular, the icaA, the icaC and the icaD genes, provides us with a rapid and accurate technique for strain characterization. However, well-established methods, such as the Congo red agar test are still needed in order to confirm the phenotypic expression in the case of possible phase-variant strains. In future, the complete knowledge of the genetic mechanisms of phenotype modulation, comprehending all regulatory genes, could permit the characterization of the isolates just by molecular means in a single step.