[Suppression of c-myc expression by interference RNA in HepG2 hepatocellular carcinoma cells].
[Suppression of c-myc expression by interference RNA in HepG2 hepatocellular carcinoma cells].
复制标题
干扰RNA抑制HepG2肝癌细胞c-myc表达的研究
DOI：
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发表时间：
2004
期刊：
影响因子：
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通讯作者：
Zong
中科院分区：
文献类型：
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作者：
Yang Xu;Yihua Wang;Ji;Jiye Ye;Hongxia Zhu;Ning;Xing;Zong
目的
研究RNA干扰（RNAi）对肝癌细胞株HepG 2中c-myc基因表达的抑制作用。
方法
构建靶向c-myc基因的小干扰RNA（siRNA）表达载体（psilencer-c-myc），脂质体介导转染HepG 2细胞，以未转染的载体作为对照（mock）。用定量PCR和Western blot检测c-myc mRNA和蛋白的表达。流式细胞仪和免疫荧光显微镜检测转染细胞的凋亡。
结果
psilencer-c-myc转染HepG 2细胞后，c-myc mRNA和蛋白的表达均受到抑制，抑制率为67%。转染后的HepG 2细胞出现凋亡。
结论
转染siRNA的HepG 2细胞中c-myc基因在转录和翻译水平的表达明显受到抑制，这可能与诱导细胞凋亡有关。
OBJECTIVE
To study the inhibitory effect of RNA interference (RNAi) on c-myc expression in hepatocellular carcinoma cell line, HepG2.
METHODS
Expression vector of c-myc gene-targeting small interference RNA (siRNA) was constructed (psilencer-c-myc) and transfected into HepG2 cells by lipofectamine, and the unloaded vector was used as control (mock). The expression of c-myc mRNA and protein was identified by quantitive PCR and Western blot. Apoptosis of the transfected cells was examined by flow cytometry and immunofluorescent microscopy.
RESULTS
After HepG2 cells were transfected with psilencer-c-myc, the expression of c-myc mRNA and protein was suppressed with an inhibition rate of 67% compared with the mock-transfected cells. Apoptosis was identified in the transfected HepG2 cells.
CONCLUSION
The expression of c-myc at transcriptional and translational levels in HepG2 cells transfected with siRNA is markedly inhibited, which may be associated with the induction of apoptosis.