The Cytotoxicity Mechanism of 6-Shogaol-Treated HeLa Human Cervical Cancer Cells Revealed by Label-Free Shotgun Proteomics and Bioinformatics Analysis.
The Cytotoxicity Mechanism of 6-Shogaol-Treated HeLa Human Cervical Cancer Cells Revealed by Label-Free Shotgun Proteomics and Bioinformatics Analysis.
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通过无标记鸟枪蛋白质组学和生物信息学分析揭示 6-Shogaol 处理的 HeLa 人宫颈癌细胞的细胞毒性机制。
DOI：
10.1155/2012/278652
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发表时间：
2012
期刊：
影响因子：
--
通讯作者：
Li P
中科院分区：
文献类型：
--
作者：
Liu Q;Peng YB;Qi LW;Cheng XL;Xu XJ;Liu LL;Liu EH;Li P
宫颈癌是世界上最常见的癌症之一。6-Shogaol是一种天然化合物，分离自生姜的根茎。在本文中，我们证明6-姜烯酚诱导人宫颈癌HeLa细胞凋亡和G2/M期阻滞。内质网应激和线粒体途径参与了6-姜烯酚介导的细胞凋亡。随后提出了基于液相色谱芯片四极杆飞行时间质谱法的无标记策略的蛋白质组学分析，以非靶偏置的方式识别细胞蛋白质中响应于6-姜烯酚处理的分子变化。HeLa细胞经15 μM 6-姜烯酚处理24 h后，共发现287个蛋白质的差异表达。通过多种分析软件对显著改变的蛋白质进行功能途径分析。免疫抑制通路分析（IPA）表明，14-3-3信号通路是6-姜烯酚诱导的细胞凋亡和G2/M期阻滞的主要途径。综上所述，本研究通过鸟枪法蛋白质组学和生物信息学分析，建立了一种无偏的蛋白质分析策略。观察到的数据提供了6-姜烯酚处理的HeLa细胞蛋白质组的全面分析，并揭示了与其抗癌机制相关的蛋白质改变。
Cervical cancer is one of the most common cancers among women in the world. 6-Shogaol is a natural compound isolated from the rhizome of ginger (Zingiber officinale). In this paper, we demonstrated that 6-shogaol induced apoptosis and G2/M phase arrest in human cervical cancer HeLa cells. Endoplasmic reticulum stress and mitochondrial pathway were involved in 6-shogaol-mediated apoptosis. Proteomic analysis based on label-free strategy by liquid chromatography chip quadrupole time-of-flight mass spectrometry was subsequently proposed to identify, in a non-target-biased manner, the molecular changes in cellular proteins in response to 6-shogaol treatment. A total of 287 proteins were differentially expressed in response to 24 h treatment with 15 μM 6-shogaol in HeLa cells. Significantly changed proteins were subjected to functional pathway analysis by multiple analyzing software. Ingenuity pathway analysis (IPA) suggested that 14-3-3 signaling is a predominant canonical pathway involved in networks which may be significantly associated with the process of apoptosis and G2/M cell cycle arrest induced by 6-shogaol. In conclusion, this work developed an unbiased protein analysis strategy by shotgun proteomics and bioinformatics analysis. Data observed provide a comprehensive analysis of the 6-shogaol-treated HeLa cell proteome and reveal protein alterations that are associated with its anticancer mechanism.
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影响因子：
14.9
作者：
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