Mobility-Selected Ion Trapping and Enrichment Using Structures for Lossless Ion Manipulations.
Mobility-Selected Ion Trapping and Enrichment Using Structures for Lossless Ion Manipulations.
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DOI：
10.1021/acs.analchem.5b03910
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发表时间：
2016-02-02
影响因子：
7.4
通讯作者：
Smith RD
中科院分区：
文献类型：
--
作者：
Chen TC;Ibrahim YM;Webb IK;Garimella SV;Zhang X;Hamid AM;Deng L;Karnesky WE;Prost SA;Sandoval JA;Norheim RV;Anderson GA;Tolmachev AV;Baker ES;Smith RD
离子迁移谱（IMS）与质谱（MS）的集成以及在IMS-MS测量中捕获离子的能力对于执行反应、积累离子和增加分析测量灵敏度是非常重要的。无损离子操纵结构（SLIM）的发展为离子操纵提供了一种扩展和更有效的方式，同时为许多更复杂的操纵序列提供了机会。在这里，我们展示了离子分离和捕获模块和基于SLIM的方法，该SLIM由线性迁移率离子漂移区、开关/三通和捕获区组成，该捕获区允许迁移率分离的物种的隔离和积累。SLIM开关/三通和陷阱的操作和优化进行了描述和演示，用于富集低丰度离子。使用SLIM阱的捕获/积累事件的数量观察到离子强度的线性改善，说明其用于增强低丰度或目标物种的灵敏度的潜力。
The integration of ion mobility spectrometry (IMS) with mass spectrometry (MS) and the ability to trap ions in IMS-MS measurements is of great importance for performing reactions, accumulating ions, and increasing analytical measurement sensitivity. The development of Structures for Lossless Ion Manipulations (SLIM) offers the potential for ion manipulations in an extended and more effective manner, while opening opportunities for many more complex sequences of manipulations. Here, we demonstrate an ion separation and trapping module and a method based upon SLIM that consists of a linear mobility ion drift region, a switch/tee and a trapping region that allows the isolation and accumulation of mobility-separated species. The operation and optimization of the SLIM switch/tee and trap are described and demonstrated for the enrichment of the low abundance ions. A linear improvement in ion intensity was observed with the number of trapping/accumulation events using the SLIM trap, illustrating its potential for enhancing the sensitivity of low abundance or targeted species.