Influence of age at drinking onset on the alcohol deprivation effect and stress-induced drinking in female rats
Influence of age at drinking onset on the alcohol deprivation effect and stress-induced drinking in female rats
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DOI：
10.1016/j.pbb.2006.10.004
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发表时间：
2007-02-01
影响因子：
3.6
通讯作者：
Spanagel, Rainer
中科院分区：
文献类型：
--
作者：
Fuellgrabe, Marc W.;Vengeliene, Valentina;Spanagel, Rainer
我们最近观察到，自青春期以来饮酒的雄性大鼠对饮酒的应激反应增加。因此，在饮酒开始的早期年龄可以诱导增强的压力诱导的饮酒在雄性大鼠。然而，目前还不知道雌性大鼠是否也有类似的反应。因此，我们比较了两个雌性Wistar大鼠组的饮酒行为-一个在青春期获得酒精消费（青少年组），另一个在成年期获得饮酒（成年组），在一个长期自愿饮酒的模型中反复剥夺和压力阶段。此外，我们研究了饮酒开始时的年龄对阿坎酸治疗效果的影响。在29周的观察期内，39只31日龄（青少年）和71日龄（成年）雌性Wistar大鼠可自由饮水以及5%和20%乙醇溶液。在获得酒精8周后引入了14天的剥夺阶段，以测量酒精剥夺效应（ADE）。在15周和25周的酒精接触后，所有动物分别连续3天进行强迫游泳和电击脚应激。在获得酒精29周后，所有动物经历第二剥夺阶段，并在阿坎酸（200 mg/kg）或载体治疗下测量随后的ADE。在第一个剥夺阶段之前的饮酒在动物组之间没有差异。然而，第一个ADE的表达在成年雌性大鼠中更明显，并且在成年组的剩余实验中，酒精摄入量保持增加。与成年组相比，反复游泳应激和足部电击应激都使青少年组的乙醇消耗量增加更明显。阿坎酸减少了成年雌性大鼠组的复发样饮酒。但对青少年组的ADE无影响。总之，在青春期开始饮酒的雌性大鼠可能更容易受到压力引起的饮酒的影响。青少年饮酒也可能导致对阿坎酸的反应降低。(c)2006年爱思唯尔公司All rights reserved.
We have recently observed increased stress responsiveness with regard to alcohol consumption in male rats that consumed alcohol since their adolescent period. Thus, early age at drinking onset can induce enhanced stress-induced alcohol drinking in male rats. However, it is not known whether female rats respond in a similar way. Therefore, we compared the drinking behavior of two female Wistar rat groups - one that acquired alcohol consumption during adolescence (adolescent group) and the other that acquired their drinking during adulthood (adult group) in a model of long-term voluntary alcohol drinking with repeated deprivation and stress phases. Furthermore, we studied the influence of age at drinking onset on the efficacy of acamprosate treatment. Thirty-nine female Wistar rats aged 31 days (adolescents) and 71 days (adults) were given ad libitum access to water, as well as to 5% and 20% ethanol solutions during an observation period of 29 weeks. A deprivation phase of 14 days was introduced following 8 weeks of access to alcohol in order to measure the alcohol deprivation effect (ADE). After 15 and 25 weeks of alcohol access, all animals were subjected for 3 consecutive days of forced swim and electric foot-shock stress, respectively. After 29 weeks of access to alcohol all animals underwent a second deprivation phase and the subsequent ADE was measured either under acamprosate (200 mg/kg) or vehicle treatment. Drinking before the first deprivation phase was not different between animal groups. However, the expression of the first ADE was more pronounced in adult female rats and alcohol intake stayed increased for the remainder of the experiment in the adult group. Both repeated swim stress and foot-shock stress produced a more pronounced increase in ethanol consumption in the adolescent group compared to the adult group. Acamprosate reduced relapse-like drinking in the adult female rat group. However, it had no effect on the ADE in the adolescent group. In conclusion, female rats that initiate alcohol consumption during adolescence might be more susceptible to stress-induced alcohol consumption. Adolescent alcohol drinking might also result in a reduced response to acamprosate. (c) 2006 Elsevier Inc. All rights reserved.