Identification of rat mammary tumor-1 gene (RMT-1), which is highly expressed in rat mammary tumors.
Identification of rat mammary tumor-1 gene (RMT-1), which is highly expressed in rat mammary tumors.
复制标题
大鼠乳腺肿瘤-1基因（RMT-1）的鉴定，该基因在大鼠乳腺肿瘤中高表达。
DOI：
10.1016/s0304-3835(01)00668-1
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发表时间：
2001
期刊：
影响因子：
9.7
通讯作者：
Nandi,S
中科院分区：
文献类型：
--
作者：
Chiou,S;Yoo,J;Loh,KC;Guzman,RC;Gopinath,GR;Rajkumar,L;Chou,YC;Yang,J;Popescu,NC;Nandi,S
早期足月妊娠可永久性降低女性患乳腺癌的风险。产大鼠和小鼠也不易发生化学致癌。因此，对初产大鼠和产大鼠乳腺差异的研究将为足月早期妊娠的保护作用提供有价值的信息。在本报告中，我们检查了基因表达模式，从处女和分娩的刘易斯大鼠乳腺。利用差分显示技术，分离到一个新的4.2 kb cDNA，命名为大鼠乳腺肿瘤-1 （RMT-1）。对RMT-1的Northern blot分析显示，RMT-1在大鼠乳腺发育的青春期前和青春期表达较高，而在成熟的初产大鼠乳腺中表达下调。RMT-1在大鼠乳腺癌中的表达均高于未孕大鼠和已产大鼠的乳腺。在Northern blot敏感性水平上，RMT-1仅在乳腺中表达。Northern blot分析还显示，该基因在74%的n -甲基亚硝基脲（MNU）诱导的乳腺癌中表达，而在MNU诱导的其他器官肿瘤中未发现。对RMT-1基因结构的分析表明，该基因由5个外显子组成，长度为5.9 kb。利用荧光原位杂交技术，将该基因定位于大鼠1号染色体q43 - 51带。目前的数据表明，RMT-1的高表达与大鼠乳腺癌的发生有关，或者RMT-1表达的降低与化学诱导乳腺癌的产次难治性有关。然而，RMT-1基因是否在这些过程中发挥功能作用仍有待研究。
Full-term pregnancy early in life results in a permanent reduction in lifetime breast cancer risk in women. Parous rats and mice are also refractory to chemical carcinogenesis. Therefore, investigation of the differences between mammary glands from virgin and parous rats would provide valuable information regarding the protective effects of early full-term pregnancy. In this report, we examined the gene expression patterns in mammary glands from virgin and parous Lewis rats. Using differential display technology, a novel 4.2 kb cDNA, designated rat mammary tumor-1 (RMT-1) was isolated. Northern blot analysis of RMT-1 showed that RMT-1 expression was higher in the pre-pubertal and pubertal stages during rat mammary gland development while it was down-regulated in mammary glands from mature virgin and parous rats. RMT-1 expression was highest in rat mammary cancers compared with either the mammary glands of virgin or parous rats. At the Northern blot sensitivity level, RMT-1 expression was found only in the mammary gland. Northern blot analysis also showed that the expression of this gene was found in 74% of N-methyl-nitrosourea (MNU)-induced mammary cancers while it was not found in MNU-induced cancers from other organs. The examination of the RMT-1 gene structure revealed that it consists of five exons spanning 5.9 kb. Using fluorescence in situ hybridization, the gene was localized on rat chromosome 1 band q 43–51. The present data show that there is a correlation between high RMT-1 expression and rat mammary carcinogenesis or decreased RMT-1 expression and parity associated refractoriness to chemically induced mammary carcinogenesis. However, whether or not RMT-1 gene has a functional role in these processes remains to be investigated.