Spontaneous Mass and Charge Losses from Single Multi-Megadalton Ions Studied by Charge Detection Mass Spectrometry
Spontaneous Mass and Charge Losses from Single Multi-Megadalton Ions Studied by Charge Detection Mass Spectrometry
复制标题
通过电荷检测质谱研究单个多兆道尔顿离子的自发质量和电荷损失
DOI：
--
复制
发表时间：
2017
影响因子：
3.2
通讯作者：
M. Jarrold
中科院分区：
文献类型：
--
作者：
David Z. Keifer;Andrew W. Alexander;M. Jarrold
用电荷检测质谱（CDMS）捕获单个B病毒（HBV）衣壳3s，观察到单个多兆道尔顿离子的自发质量和电荷损失。离子阱中单个离子振荡频率的逐渐增加主要归因于质量损失（可能是溶剂、水和/或盐）。对于4 MDa HBV T = 4衣壳，在3秒捕获期期间的总质量损失在约20 kDa处达到峰值。离散的频率下降打断了振荡频率的逐渐增加。这些下降是由于突然失去电荷造成的。在大多数情况下，单个正电荷随着一定质量（平均约1000 Da）而沿着丢失。超过40%的捕获离子发生电荷损失。它通常发生在捕获事件的开始附近，并且优先发生在具有强电场的陷阱区域，表明外部电场促进电荷损失。这一过程可能导致兆道尔顿离子经常出现的m/z分辨率降低。
图形摘要
AbstractSpontaneous mass and charge losses from individual multi-megadalton ions have been observed with charge detection mass spectrometry (CDMS) by trapping single hepatitis B virus (HBV) capsids for 3 s. Gradual increases in the oscillation frequency of single ions in the ion trap are attributed mainly to mass loss (probably solvent, water, and/or salt). The total mass lost during the 3 s trapping period peaks at around 20 kDa for 4 MDa HBV T = 4 capsids. Discrete frequency drops punctuate the gradual increases in the oscillation frequencies. The drops are attributed to a sudden loss of charge. In most cases a single positive charge is lost along with some mass (on average around 1000 Da). Charge loss occurs for over 40% of the trapped ions. It usually occurs near the beginning of the trapping event, and it occurs preferentially in regions of the trap with strong electric fields, indicating that external electric fields promote charge loss. This process may contribute to the decrease in m/z resolution that often occurs with megadalton ions.
Graphical Abstractᅟ
影响因子：
7.4
作者：
Hogan, Christopher J., Jr.;Carroll, James A.;Rohrs, Henry W.;Biswas, Pratim;Gross, Michael L.
通讯作者：
Gross, Michael L.
影响因子：
15
作者：
Hogan, Christopher J., Jr.;Carroll, James A.;Gross, Michael L.
通讯作者：
Gross, Michael L.