Release of ANF, proANF 1-98, and proANF 31-67 from isolated rat atria by atrial distension.
Release of ANF, proANF 1-98, and proANF 31-67 from isolated rat atria by atrial distension.
复制标题
通过心房扩张，从离体大鼠心房中释放 ANF、proANF 1-98 和 proANF 31-67。
DOI：
10.1152/ajpheart.1991.260.6.h1774
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发表时间：
1991
期刊：
影响因子：
--
通讯作者：
Vesely,DL
中科院分区：
文献类型：
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作者：
Dietz,JR;Nazian,SJ;Vesely,DL
本研究的目的是确定是否心钠素和NH 2-末端的心钠素激素原分泌的同时在响应于心房扩张在离体灌注大鼠心房。实验在用改良Krebs缓冲液灌注的起搏左心房中进行。在一组（n = 9）隔离心房中，心房压从基线水平2 mmHg升高至8-9 mmHg，持续60分钟（扩张），然后恢复至2 mmHg，持续60分钟。在第二组心房（n = 6）中，在整个实验期间，心房压力保持在约2 mmHg。在心房压力升高前的3个10分钟内，ANF分泌平均为100 pg/min，当心房压力升高时，ANF分泌增加到600-800 pg/min（P <0.001）。proANF 31-67肽的分泌从扩张前即刻的约100 pg/min的值增加到扩张期间超过200 pg/min的峰值（P小于0.01）。proANF 1-98的分泌从扩张前三个时期的平均1.25 ng/min增加到扩张期间的峰值2.5 ng/min（P <0.01）。这些数据表明，心钠素和NH 2-末端的心钠素激素似乎是同时分泌的孤立起搏心房。
The purpose of the present study was to determine if ANF and the NH2-terminus of the ANF prohormone are secreted simultaneously in response to atrial distension in isolated perfused rat atria. The experiments were conducted in paced left atria perfused with a modified Krebs buffer. Atrial pressure was increased from a baseline level of 2 mmHg to 8-9 mmHg for 60 minutes (distension) and then returned to 2 mmHg for 60 minutes in one group (n = 9) of isolated atria. In a second group of atria (n = 6), atrial pressure was maintained at approximately 2 mmHg throughout the experimental period. ANF secretion averaged 100 pg/min during the three 10-minute periods immediately preceding the increased atrial pressure and increased to 600-800 pg/min (P less than 0.001) when atrial pressure was raised. Secretion of the proANF 31-67 peptide increased from a value of approximately 100 pg/min immediately prior to distension to a peak of over 200 pg/min during distension (P less than 0.01). Secretion of proANF 1-98 increased from an average of 1.25 ng/min during the three periods immediately prior to distension to a peak of 2.5 ng/min during distension (P less than 0.01). These data indicate that ANF and the NH2-terminus of the ANF prohormone appear to be simultaneously secreted by isolated paced atria.