Molecular Epidemiology of NDM-1-Producing Enterobacteriaceae and Acinetobacter baumannii Isolates from Pakistan
Molecular Epidemiology of NDM-1-Producing Enterobacteriaceae and Acinetobacter baumannii Isolates from Pakistan
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DOI：
10.1128/aac.02425-14
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发表时间：
2014-09-01
影响因子：
4.9
通讯作者：
Sidjabata, Hanna E.
中科院分区：
文献类型：
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作者：
Sartor, Anna L.;Raza, Muhammad W.;Sidjabata, Hanna E.
对来自巴基斯坦2家医院的66株NDM菌株进行了分子流行病学调查，并用重复序列聚合酶链式反应(Rep-PCR)确定了它们的遗传亲缘关系。还进行了基于PCR的复制子分型和对编码碳青霉烯酶、其他β-内酰胺酶和16S甲基酶的抗生素耐药基因的筛选。REP-PCR提示阴沟肠杆菌和大肠杆菌的克隆性传播。鉴定出多种质粒复制子类型，其中以不相容A/C组(INCA/C)最常见(78%)。产NDM的肠杆菌科细菌中有81%含有16S甲基酶编码基因。
The molecular epidemiology of 66 NDM-producing isolates from 2 Pakistani hospitals was investigated, with their genetic relatedness determined using repetitive sequence-based PCR (Rep-PCR). PCR-based replicon typing and screening for antibiotic resistance genes encoding carbapenemases, other beta-lactamases, and 16S methylases were also performed. Rep-PCR suggested a clonal spread of Enterobacter cloacae and Escherichia coli. A number of plasmid replicon types were identified, with the incompatibility A/C group (IncA/C) being the most common (78%). 16S methylase-encoding genes were coharbored in 81% of NDM-producing Enterobacteriaceae.