TANDEMLY ARRANGED GENE CLUSTERS OF MALARIAL PARASITES THAT ARE HIGHLY CONSERVED AND TRANSCRIBED
TANDEMLY ARRANGED GENE CLUSTERS OF MALARIAL PARASITES THAT ARE HIGHLY CONSERVED AND TRANSCRIBED
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DOI：
10.1016/0166-6851(87)90056-9
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发表时间：
1987-01-15
影响因子：
1.5
通讯作者：
ARASU, P
中科院分区：
文献类型：
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作者：
VAIDYA, AB;ARASU, P
从啮齿类疟原虫约氏疟原虫基因组文库中分离出一个含有5.8kb EcoRI片段的分子克隆。约氏疟原虫基因组包含约150个拷贝的该序列，占DNA的近3%。这些序列以头对尾的构型串联排列，重复序列的单位长度为5.8kb。约氏疟原虫1.6 ~ 0.3kb的多聚（A+）RNA可被5.8kb克隆识别。另外五种疟疾寄生虫（夏氏疟原虫、伯氏疟原虫、恶性疟原虫、诺氏疟原虫和食蟹猴疟原虫）含有串联重复序列阵列，其具有5.8 kb的相同单位长度，易于与从约氏疟原虫克隆的序列杂交。
A molecular clone containing a 5.8 kb Eco RI fragment was isolated from a genomic library of the rodent malarial parasite Plasmodium yoelii. The P. yoelligenome contains about 150 copies of this sequence, making up almost 3% of the DNA. These sequences are tandemly arrayed in head-to-tail configurations with the unit length of the repeat being 5.8 kb. Several poly(A+) RNAs of P. yoelii ranging from 1.6 to 0.3 kb are recognized by the 5.8 kb clone. Five additional species of malarial parasites (P. chabaudi, P. berghei, P. falciparum, P. knowlesi, and P. cynomolgi) contain tandemly repeated arrays of sequences having the same unit length of 5.8 kb, which readily hybridize to the sequence cloned from P. yoelii.