The Zur of Xanthomonas campestris Is Involved in Hypersensitive Response and Positively Regulates the Expression of the hrp Cluster Via hrpX But Not hrpG
The Zur of Xanthomonas campestris Is Involved in Hypersensitive Response and Positively Regulates the Expression of the hrp Cluster Via hrpX But Not hrpG
复制标题
野油菜黄单胞菌的 Zur 参与过敏反应并通过 hrpX 但不通过 hrpG 正向调节 hrp 簇的表达
DOI：
10.1094/mpmi-22-3-0321
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发表时间：
2009-03-01
影响因子：
3.5
通讯作者：
Tang, Ji-Liang
中科院分区：
文献类型：
--
作者：
Huang, Dong-Liang;Tang, Dong-Jie;Tang, Ji-Liang
在细菌中，Zur是锌稳态的关键调节剂。我们以前的工作表明，在植物病原体野油菜黄单胞菌致病变种（Xanthomonascampestris pv.）除了调节锌的体内平衡外，Zur对于完全毒力是必需的。在这里，我们证明了X。野油菜致病变种campestris Zur参与过敏反应（hypersensitivesresponse，HR），并正调控hrpA到hrpF操纵子和hrpX的转录，但不调控hrpG的转录。在zur突变体中组成型表达hrpX而非hrpG可以绕过Zur对hrpA至hrpF操纵子表达和野生型HR诱导的需要，表明Zur通过hrpX控制hrp簇的表达。启动子-gusA报告基因和半定量逆转录聚合酶链反应分析表明，HrpG控制hrpX的表达，HrpX调节X中所有六个hrp操纵子（hrpA至hrpF）的表达。野油菜致病变种油菜
In bacteria, Zur is a key regulator for zinc homeostasis. Our previous work has shown that, in the phytopathogen Xanthomonas campestris pv. campestris, in addition to regulating zinc homeostasis, Zur is essential for full virulence. Here, we demonstrate that the X. campestris pv. campestris Zur is involved in hypersensitive response (HR) and positively regulates the transcription of hrpA to hrpF operons and hrpX but not hrpG. Constitutively expressing hrpX but not hrpG in the zur mutant could bypass the requirement of Zur for the expression of hrpA to hrpF operons and the induction of wild-type HR, indicating that Zur controls the expression of hrp cluster via hrpX. Promoter-gusA reporter and semiquantitative reverse-transcription polymerase chain reaction analyses revealed that HrpG controls the expression of hrpX and HrpX regulates the expression of all the six hrp operons (hrpA to hrpF) in X. campestris pv. campestris.