ROLE OF BACTERIAL ASSOCIATION WITH KUPFFER CELLS IN OCCURRENCE OF ENDOGENOUS SYSTEMIC BACTEREMIA
ROLE OF BACTERIAL ASSOCIATION WITH KUPFFER CELLS IN OCCURRENCE OF ENDOGENOUS SYSTEMIC BACTEREMIA
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DOI：
10.1128/iai.59.1.289-294.1991
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发表时间：
1991-01-01
影响因子：
3.1
通讯作者：
YAMAGUCHI, K
中科院分区：
文献类型：
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作者：
HIRAKATA, Y;TOMONO, K;YAMAGUCHI, K
免疫功能低下宿主的菌血症通常由其内源性肠道植物群引起。 我们通过给常规和无特定病原体的小鼠施用环磷酰胺和氨苄青霉素来产生实验性内源性菌血症。 常规小鼠的菌血症和死亡率的频率显著高于无特定病原体的小鼠。 铜绿假单胞菌是引起常规小鼠全身性菌血症的主要病原体，并与高死亡率相关。 摩氏摩根菌引起全身性菌血症在传统和特定病原体无小鼠。 相反，大肠杆菌、肠球菌或其他菌种最常仅引起门脉菌血症。 为了确定全身性菌血症发生的机制，我们使用从菌血症小鼠中分离的几种细菌菌株，研究了小鼠中细菌血液清除率及其与小鼠枯否细胞的相关性。 引起门脉菌血症的微生物的血液清除率和分离的枯否细胞与细菌结合的能力显著高于引起全身菌血症的微生物。 角叉菜胶处理小鼠的血液清除率与正常小鼠无显著差异。 此外，在4 ℃下的缔合与在37 ℃下的缔合没有不同。 结果表明，血液中细菌的清除反映了细菌对枯否细胞的粘附，并且细菌对枯否细胞的抗性在该动物模型中压倒性全身菌血症的发生中起重要作用。
Bacteremia in immunocompromised hosts often arises from their endogenous intestinal flora. We produced experimental endogenous bacteremia by administering cyclophosphamide and ampicillin to conventional and specific-pathogen-free mice. The frequencies of bacteremia and mortality in the conventional mice were significantly higher than for the specific-pathogen-free mice. Pseudomonas aeruginosa was the major pathogen causing systemic bacteremia in conventional mice and was associated with a high mortality rate. Morganella morganii caused systemic bacteremia in both conventional and specific-pathogen-free mice. In contrast, Escherichia coli, enterococci, or other species most often caused portal bacteremia only. To determine the mechanism of occurrence of systemic bacteremia, we investigated bacterial blood clearance in mice and association with murine Kupffer cells, using several bacterial strains isolated from mice with bacteremia. Blood clearance rates and the abilities of isolated Kupffer cells to associate with bacteria were significantly greater for the organisms causing portal bacteremia than for those causing systemic bacteremia. There were no significant differences between the blood clearance rates in carrageenan-treated mice and that in normal mice. Moreover, association at 4-degrees-C was not different from that at 37-degrees-C. The results suggest that blood clearance of bacteria reflects bacterial adherence to Kupffer cells and that the resistance of bacteria to association with Kupffer cells plays an important role in the occurrence of overwhelming systemic bacteremia in this animal model.