Transcription regulation by initiating NTP concentration: RRNA synthesis in bacteria
Transcription regulation by initiating NTP concentration: RRNA synthesis in bacteria
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DOI：
10.1126/science.278.5346.2092
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发表时间：
1997-12-19
期刊：
影响因子：
56.9
通讯作者：
Gourse, RL
中科院分区：
文献类型：
--
作者：
Gaal, T;Bartlett, MS;Gourse, RL
启动子的序列不仅决定了它与RNA聚合酶形成复合物的效率，还决定了启动转录所需的三磷酸核苷（NTP）的浓度。大肠杆菌核糖体RNA（rrn P1）启动子需要高起始NTP浓度来有效转录，因为它们与RNA聚合酶形成异常短暂的复合物;需要高起始NTP浓度[腺苷或三磷酸鸟苷（ATP或GTP），取决于rrn P1启动子]来结合并稳定开放复合物。ATP和GTP浓度，因此rrn P1启动子活性，随着生长速度的增加。因为核糖体RNA转录决定核糖体合成的速率，所以通过NTP浓度对核糖体RNA转录的控制为核糖体合成的生长速率依赖性控制和稳态调节提供了分子解释。
The sequence of a promoter determines not only the efficiency with which it forms a complex with RNA polymerase, but also the concentration of nucleoside triphosphate (NTP) required for initiating transcription. Escherichia coli ribosomal RNA (rrn PI) promoters required high initiating NTP concentrations for efficient transcription because they form unusually short-lived complexes with RNA polymerase; high initiating NTP concentrations [adenosine or guanosine triphosphate (ATP or GTP), depending on the rrn P1 promoter] are needed lo bind to and stabilize the open complex. ATP and GTP concentrations, and therefore rrn P1 promoter activity, increase with growth rate. Because ribosomal RNA transcription determines the rate of ribosome synthesis, the control of ribosomal RNA transcription by NTP concentration provides a molecular explanation for the growth rate-dependent control and homeostatic regulation of ribosome synthesis.