Rapid diaphragm atrophy following cervical spinal cord hemisection.
Rapid diaphragm atrophy following cervical spinal cord hemisection.
复制标题
DOI：
10.1016/j.resp.2013.12.006
复制
发表时间：
2014-02-01
影响因子：
2.3
通讯作者：
Fuller, D. D.
中科院分区：
文献类型：
--
作者：
Gill, L. C.;Ross, H. H.;Lee, K. Z.;Gonzalez-Rothi, E. J.;Dougherty, B. J.;Judge, A. R.;Fuller, D. D.
颈椎（C2）半裂（C2Hx）破坏同侧球脊髓对膈核的输入，用于研究急性脊髓损伤后膈肌的可塑性。我们假设C2Hx会导致同侧半膈快速萎缩，并增加蛋白水解生物标志物的mRNA表达。雄性Sprague-Dawley大鼠于C2Hx后1或7天采集膈肌组织。组织学分析显示，损伤后1天（而不是7天），同侧半膈I型和IIa型纤维的横截面积（CSA）减少。然而，IIb/x型纤维在第1天和第7天的CSA均有所降低。为了研究c2hx诱导横膈膜萎缩的机制，使用靶向基因阵列筛选骨骼肌肌病和肌肉发生相关基因的mRNA变化；随后进行qRT-PCR验证。横膈膜基因表达的变化表明，C2Hx后立即启动了深刻的肌可塑性，包括蛋白水解和肌生成途径的激活。我们的结论是，C2Hx后膈肌立即发生肌增生反应，同侧肌纤维在1天内发生萎缩。
A cervical (C2) hemilesion (C2Hx), which disrupts ipsilateral bulbospinal inputs to the phrenic nucleus, was used to study diaphragm plasticity after acute spinal cord injury. We hypothesized that C2Hx would result in rapid atrophy of the ipsilateral hemidiaphragm and increases in mRNA expression of proteolytic biomarkers. Diaphragm tissue was harvested from male Sprague-Dawley rats at 1 or 7 days following C2Hx. Histological analysis demonstrated a reduction in cross-sectional area (CSA) of type I and IIa fibers in the ipsilateral hemidiaphragm at 1 but not 7 days post-injury. Type IIb/x fibers, however, had reduced CSA at both 1 and 7 days. To examine the mechanisms of C2Hx-induced diaphragm atrophy, a targeted gene array was used to screen mRNA changes for genes associated with skeletal muscle myopathy and myogenesis; this was followed by qRT-PCR validation. Changes in diaphragm gene expression suggested that profound myoplasticity is initiated immediately following C2Hx including activation of both proteolytic and myogenic pathways. We conclude that an immediate myoplastic response occurs in the diaphragm after C2Hx with atrophy occurring in ipsilateral myofibers within 1 day.
登录
查看更多内容
影响因子：
3.3
作者：
KATAGIRI, M;YOUNG, RN;EASTON, PA
通讯作者：
EASTON, PA
DOI：
10.1016/j.jtcvs.2011.02.025
发表时间：
2011-11-01
影响因子：
6
作者：
Huang, Tseng-Tien;Deoghare, Harsha V.;Martin, A. Daniel
通讯作者：
Martin, A. Daniel
DOI：
10.1164/rccm.2202005
发表时间：
2002-08-15
影响因子：
24.7
作者：
Reid, MB;Lännergren, J;Westerblad, H
通讯作者：
Westerblad, H
影响因子：
3.3
作者：
Argadine, Heather M.;Hellyer, Nathan J.;Sieck, Gary C.
通讯作者：
Sieck, Gary C.
影响因子：
3.3
作者：
Powers, SK;Shanely, RA;Dodd, SL
通讯作者：
Dodd, SL