Gd-DTPA MR detection of blood-brain barrier opening in rats after hyperosmotic shock.
Gd-DTPA MR detection of blood-brain barrier opening in rats after hyperosmotic shock.
复制标题
Gd-DTPA MR检测大鼠高渗休克后血脑屏障的开放情况。
DOI：
10.1097/00004728-199307000-00008
复制
发表时间：
1993
影响因子：
1.3
通讯作者：
Stevenson,DK
中科院分区：
文献类型：
--
作者：
Rhine,WD;Benaron,DA;Enzmann,DR;Chung,C;Gonzales-Mendez,R;Sayre,JR;Stevenson,DK
目的：新生儿血脑屏障（BBB）开口检测需要有创性方法，临床不适用。我们着手开发一种非侵入性方法来检测这种开口。材料和方法：采用MRI和Gd-DTPA对比研究Wistar大鼠注射高渗溶液前后的屏障打开情况。阿拉伯糖经右颈动脉给药，造成单侧屏障打开；经尾静脉输注尿素，形成双侧开口；对照组接受生理盐水。随后，所有动物均经尾静脉注射Gd-DTPA。结果：接受颈动脉高渗注射的动物在同侧大脑半球出现信号增高；接受静脉高渗注射的患者双侧信号增加。在使用高渗剂或生理盐水对照组之前，没有发现类似的增加。在这两种情况下，即使在没有半球控制的情况下，也可以使用Gd-DTPA在屏障内和屏障外结构之间的相对划分来检测屏障打开。结论：Gd-DTPA对比MRI可以无创检测大鼠血脑屏障开口。
Objective: Detection of blood-brain barrier (BBB) opening in neonates has required invasive methods not clinically applicable. We set out to develop a noninvasive approach to detect such opening.Materials and Methods: Wistar rats were studied using MRI with Gd-DTPA contrast before and after injection of hyperosmotic solutions known to produce barrier opening. Arabinose was given via right carotid artery to produce unilateral barrier opening; urea was given via tail vein to produce bilateral opening; controls received normal saline. Next, all animals received Gd-DTPA via tail vein.Results: Animals receiving carotid hyperosmotic injections showed increased signal in the ipsilateral brain hemisphere; those receiving venous hyperosmotic injections showed increased signal bilaterally. Similar increases were not found prior to administration of hyperosmotic agent or in saline controls. In both cases, barrier opening was detectable using the relative partitioning of Gd-DTPA between intrabarrier and extrabarrier structures, even in the absence of a hemispheric control.Conclusion: We conclude that MRI with Gd-DTPA contrast allows noninvasive detection of BBB opening in the rat.