Analysis of nuclear 5,5,3'-triiodothyronine-binding capacity and tissue response in the liver of the neonatal rat.
Analysis of nuclear 5,5,3'-triiodothyronine-binding capacity and tissue response in the liver of the neonatal rat.
复制标题
新生大鼠肝脏中核 5,5,3-三碘甲状腺原氨酸结合能力和组织反应的分析。
DOI：
10.1210/endo-105-4-952
复制
发表时间：
1979
期刊：
影响因子：
4.8
通讯作者：
J. Dussault
中科院分区：
文献类型：
--
作者：
P. Coulombe;J. Ruel;J. Dussault
进行了研究，以确定肝脏对T3管理的新生大鼠，并在溶解的肝细胞核提取物的最大T3结合能力的特点。Scatchard分析表明，平衡结合常数从出生至5日龄期间的4.19 ± 0.58 × 109 M-1（平均值± SEM）逐渐增加至21-30日龄期间的平台值5.75 ± 1.02 × 109 M-1。同样，成年大鼠的最大结合容量显著大于5日龄以下大鼠（P < 0.01）。MBC在出生后21-30天达到平台期。在7、10和24日龄大鼠中进行甲状腺激素类似物置换研究。结果表明，T4、3，3 '-二碘甲腺原氨酸和rT 3以与成年动物相同的亲和力与受体结合。对线粒体肝酶α-甘油磷酸脱氢酶（α-GPD）活性的测定表明，钽（100 μg/100 g BW）诱导24 h后该酶活性增加2.0倍。
Studies were undertaken to determine the hepatic response to T3 administration in the neonatal rat and to characterize the maximum T3-binding capacity in solubilized hepatic nuclei extracts. Scatchard analysis snowed that the equilibrium association constant gradually increased from 4.19 ± 0.58 × 109 M-1 (mean ± SEM) between birth and 5 days of age to a plateau value of 5.75 ± 1.02 × 109 M-1 between 21–30 days of age. Similarly, the maximum binding capacity was significantly greater in adult rats than in rats under 5 days of age (P < 0.01). MBC reached a plateau between 21–30 days after birth. Thyroid hormone analog displacement studies were performed in 7-, 10-, and 24-day-old rats. Results indicate that T4, 3,3'-diodothyronine, and rT3 bind to the receptor with the same affinity as in the adult animal. Measurement of the activity of the mitochondrial hepatic enzyme a-glycerophosphate dehydrogenase (α-GPD) showed that Ta (100 μg/100 g BW) induced a 2.0-fold increase in the activity of the enzyme 24 h aft...