Galectin-1 Enhances Astrocytic BDNF Production and Improves Functional Outcome in Rats Following Ischemia
Galectin-1 Enhances Astrocytic BDNF Production and Improves Functional Outcome in Rats Following Ischemia
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Galectin-1 增强星形胶质细胞 BDNF 的产生并改善缺血后大鼠的功能结果
DOI：
10.1007/s11064-010-0234-z
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发表时间：
2010-11-01
影响因子：
4.4
通讯作者：
Wang, Wei
中科院分区：
文献类型：
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作者：
Qu, Wen-sheng;Wang, Yi-hui;Wang, Wei
半乳糖凝集素-1是一种内源性哺乳动物凝集素，与多种中枢神经系统疾病有关。然而，其在脑缺血中的作用仍然是难以捉摸的。在本研究中，我们研究了重组半乳糖凝集素-1对脑缺血后星形胶质细胞脑源性神经营养因子（BDNF）产生和功能恢复的影响。内源性半乳糖凝集素-1被认为是显着上调，增加星形胶质细胞BDNF的生产在体外和体内缺血条件下增加。Galectin-1剂量依赖性地促进星形胶质细胞BDNF的表达和分泌。此外，光化学脑缺血大鼠显示缺血边界区神经元凋亡减少，脑灌注半乳糖凝集素-1（1 μ g/天，7天）后功能恢复改善。这些结果表明，半乳糖凝集素-1诱导星形胶质细胞中的BDNF可能是一种有前途的干预，以减轻脑卒中后的脑损伤。
Galectin-1, an endogenous mammalian lectin, has been implicated in a variety of CNS disorders. However, its role in cerebral ischemia is still elusive. In the present study, we investigated the effect of recombinant galectin-1 on production of astrocytic brain-derived neurotrophic factor (BDNF) and functional recovery following ischemia. Endogenous galectin-1 was found to be markedly upregulated, paralleled with increased astrocytic BDNF production under ischemic conditions both in vitro and in vivo. Administration of galectin-1significantly enhanced the expression and secretion of astrocytic BDNF in dose dependent manner. Moreover, rats subjected to photochemical cerebral ischemia showed reduced neuronal apoptosis in ischemic boundary zone and improved functional recovery after brain infusion of galectin-1 (1 mu g/days, 7 days). These results suggest that induction of BDNF in astrocytes by galectin-1 may be a promising intervention to attenuate brain damage after stroke.