Liposomes carrying diatrizoate. Characterization of biophysical properties and imaging applications.
Liposomes carrying diatrizoate. Characterization of biophysical properties and imaging applications.
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携带泛影酸盐的脂质体。
DOI：
10.1097/00004424-198403000-00012
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发表时间：
1984
影响因子：
6.7
通讯作者：
A. Havron
中科院分区：
文献类型：
--
作者：
S. Seltzer;M. A. Davis;D. Adams;P. Shulkin;W. Landis;A. Havron
我们已经制备并表征了携带泛影葡胺的脂质体混悬液。囊泡是由鸡蛋卵磷脂，胆固醇和硬脂胺以4：1：1的摩尔比，并含有泛影葡胺钠在其水相。它们的尺寸最大可达2.0微米，具有多层结构。然后将这些囊泡注射到正常和携带肿瘤的大鼠，以及正常的狗和狒狒中。碘成分被证明具有延长的血池停留时间，通过网状内皮和尿组织清除，并在7天内完全排出。小鼠LD 50为2.3 g I/kg（38.5 g脂质体混悬液/kg）。泛影葡胺脂质体的成像研究显示血池、肝、脾、肾、尿和肿瘤边缘的明显和长期的对比增强。此外，血液、肝脏和脾脏浑浊程度更大，持续时间更长，比使用游离泛影葡胺中等量碘时更长。这些携带泛影葡胺的脂质体特别适用于血池和网状内皮结构的计算机断层成像。
We have prepared and characterized a suspension of liposomes carrying diatrizoate. Vesicles were made with egg lecithin, cholesterol, and stearylamine in a 4:1:1 molar ratio, and contained meglumine sodium diatrizoate in their aqueous phase. They ranged up to 2.0 microns in size and had a multilamellar structure. These vesicles were then injected into normal and tumor-bearing rats, as well as normal dogs and a baboon. The iodine component proved to have a prolonged blood pool residence time, was cleared through reticuloendothelial and urinary tissues, and was completely excreted within seven days. The LD50 in mice was 2.3 g I/kg (38.5 g of liposome suspension/kg). Imaging studies with diatrizoate-carrying liposomes demonstrated marked and prolonged contrast enhancement of blood pool, liver, spleen, kidneys, urine, and tumor rims. Furthermore, the blood, liver, and spleen opacification was greater and longer sustained than when an equivalent amount of iodine in free diatrizoate was used. These diatrizoate-carrying liposomes are particularly well suited for computed tomographic imaging of blood pool and reticuloendothelial structures.