Simultaneous determination of hemoglobin derivatives, oxygen content, oxygen capacity, and oxygen saturation in 10 microliters of whole blood.
Simultaneous determination of hemoglobin derivatives, oxygen content, oxygen capacity, and oxygen saturation in 10 microliters of whole blood.
复制标题
同时测定10微升全血中的血红蛋白衍生物、氧含量、氧容量和氧饱和度。
DOI：
10.1093/clinchem/23.7.1215
复制
发表时间：
1977
影响因子：
9.3
通讯作者：
I. Raffaele
中科院分区：
文献类型：
--
作者：
L. Rossi;M. Perella;M. Luzzana;Michele Samaja;I. Raffaele
介绍了一种同时测定10微升全血中四种血红蛋白衍生物(脱氧血红蛋白Hb、氧合血红蛋白HbO2、高铁血红蛋白Hb+和一氧化碳血红蛋白HbCO)和总氧含量的新方法。HbO2、HbCO、Hb+、总血红蛋白(HBT)和氧量的百分比也可以通过简单的计算从实验数据中得到。总分析时间为1分钟。血液用准厌氧试管中的缓冲液稀释100倍，在那里同时测量氧压(通过PO2电极)和吸光度(在497,565和620 nm处)。氧电极记录的氧压下降与脱氧血红蛋白的量成正比。HBO2、HbCO和Hb+的浓度可以从指定波长的吸光度测量中获得。这种新方法不需要使用短路光学元件，并且由于样本量要求较低，使得自动测量动脉化毛细血管血中的血红蛋白衍生物和血氧饱和度成为可能。
We describe a new method for simultaneous determination of four hemoglobin derivatives (deoxyhemoglobin, Hb; oxyhemoglobin, HbO2; methemoglobin, Hb+; and carbon monoxide hemoglobin, HbCO) and total oxygen content in 10 microliters of whole blood. Percentage HbO2, HbCO, Hb+, total hemoglobin (Hbt), and oxygen capacity can also be obtained from the experimental data by simple calculations. Total analysis time is 1 min. Blood is diluted 100-fold with a buffer contained in a quasi-anaerobic cuvette, where simultaneous measurements of oxygen pressure (by a po2 electrode) and adsorbance (at 497, 565, and 620 nm) are made. The decrease of oxygen pressure, as recorded by the oxygen electrode, is proportional to the amount of deoxyhemoglobin. The concentrations of HbO2, HbCO, and Hb+ can be obtained from absorbance measurements at the specified wavelengths. The new method eliminates the use of short-path optical cells and, due to the low sample volume requirement, makes possible the automated measurement of hemoglobin derivatives and oxygen saturation in arterialized capillary blood.