High prevalence of CsRV2 in cultured Callinectes danae: Potential impacts on soft-shell crab production in Brazil
High prevalence of CsRV2 in cultured Callinectes danae: Potential impacts on soft-shell crab production in Brazil
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CsRV2 在养殖 Callinectes danae 中的高流行：对巴西软壳蟹生产的潜在影响
DOI：
10.1016/j.jip.2022.107739
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发表时间：
2022
影响因子：
3.4
通讯作者：
James Schott, Eric
中科院分区：
文献类型：
--
作者：
Prestes dos Santos Tavares, Camila;Zhao, Mingli;Lopes Vogt, Éverton;Felipe Argenta Model, Jorge;Sommer Vinagre, Anapaula;de Assis Teixeira da Silva, Ubiratan;Ostrensky, Antonio;James Schott, Eric
螃蟹可以被各种病原微生物感染，但最具破坏性的是病毒。在美国，自然发生的Callinectes sapidus呼肠孤病毒1（CsRV 1）被认为是导致软蟹养殖中Callinectes sapidus死亡的原因。在巴西，软蟹经常使用Callinectes danae生产，它在养殖中的死亡率与C相似。sapidus。本研究调查了在巴西巴拉那州的健康和死亡的Callinectes danae中是否可以检测到CsRV 1，并评估了病毒感染与生化和行为参数之间的关系。将来自帕拉纳瓜湾（Paranaguá Bay）的丹娜（Danae）在再循环系统中保持14天，并进行每周的生化分析和反射作用死亡率预测因子（RAMP）测试。所有样本的CsRV 1 RT-qPCR检测均为阴性。然而，从一些螃蟹中提取的RNA的电泳分析显示了12条dsRNA带的模式，表明强烈感染的呼肠孤病毒与CsRV 1不同的基因组组织。该带型与C. sapidusin南里奥格兰德，巴西，暂时称为CsRV 2。死亡蟹和存活蟹的CsRV 2双链RNA的感染率无显著差异。有趣的是，CsRV 2 dsRNA的存在与肝胰腺中糖原浓度的显著降低和反射作用的降低相关。本研究的结果是对呼肠孤病毒在C. danae及其在巴西软壳蟹系统中的潜在影响。
Crabs can be infected by a variety of pathogenic micro-organisms but the most damaging are viruses. Naturally-occurring Callinectes sapidus reovirus 1 (CsRV1) is thought to contribute to mortality ofCallinectes sapidusin soft crab culture in the USA. In Brazil, soft crabs are frequently produced usingCallinectes danae, which suffers a similar rate of mortality in culture asC. sapidus. This study investigated whether CsRV1 could be detected in healthy or deadCallinectes danaefrom Paraná, Brazil and kept in captivity, we also evaluated the relationship between viral infection, and biochemical and behavioral parameters.C. danaefrom Paranaguá Bay were kept in a recirculation system for 14 days and subjected to weekly biochemical analyses and a reflex action mortality predictors (RAMP) test. RT-qPCR assays for CsRV1 were negative for all samples. However, electrophoretic analysis of extracted RNA from some crabs showed a pattern of 12 dsRNA bands that indicated intense infection by a reovirus with a genome organization different from CsRV1. The banding pattern was indistinguishable from a putative novel reovirus detected inC. sapidusin Rio Grande do Sul, Brazil, provisionally called CsRV2. The prevalence of dsRNA of CsRV2 showed no significant difference between crabs that died and survived. Interestingly, the presence of CsRV2 dsRNA was correlated with a significant reduction in glycogen concentration in hepatopancreas and a decrease in reflex action. The results obtained in this study are an early glimpse of the occurrence of reoviruses inC. danaeand their potential effects in soft-shell crab systems in Brazil.