Dynamic change in expression of LECT2 during liver regeneration after partial hepatectomy in mice
Dynamic change in expression of LECT2 during liver regeneration after partial hepatectomy in mice
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DOI：
10.2220/biomedres.28.247
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发表时间：
2007-10-01
影响因子：
1.2
通讯作者：
Suzuki, Kazuo
中科院分区：
文献类型：
--
作者：
Ohtomi, Michiko;Nagai, Hisakazu;Suzuki, Kazuo
白细胞衍生趋化因子2（LECT 2）首先从植物血凝素激活的人T细胞白血病SKW-3细胞的培养液中分离出来，并发现在人、牛和小鼠的肝脏中表达。为了进一步分析LECT 2在肝脏中的作用，我们采用免疫组织化学和原位杂交技术研究了小鼠LECT 2（mLECT 2）在部分肝切除术（PHx）后肝再生过程中的表达。在正常小鼠肝细胞中检测到小鼠LECT 2蛋白和mRNA，但在PHx后30 min，在肝组织中未检测到它们。在PHx后2 h，在中央静脉周围的肝细胞中观察到mLECT 2蛋白的表达，尽管mRNA表达水平仍然较低。在PHx后6 h，在整个肝脏中观察到大量表达mLECT 2蛋白和mRNA的肝细胞，在PHx后12 h，表达mLECT 2蛋白和mRNA的肝细胞数量进一步增加。然而，与PHx后12 h的水平相比，PHx后24 h的mLECT 2蛋白和mRNA表达水平显著降低。这些结果表明，LECT 2触发再生的早期事件，伴随着肝细胞增殖的抑制。
Leukocyte-cell-derived chemotaxin 2 (LECT2) was first isolated from the culture fluid of phytohemagglutinin-activated human T-cell leukemia SKW-3 cells and was found to be expressed in the human, bovine and murine livers. To further analyze the role of LECT2 in the liver, we investigated the expression of mouse LECT2 (mLECT2) during liver regeneration after partial hepatectomy (PHx) using immunohistochemical and in situ hybridization techniques. Mouse LECT2 protein and mRNA were detected in most hepatocytes in normal mouse; however, at 30 min after PHx, they were not detected in liver tissue. At 2 h after PHx, expression of mLECT2 protein was seen in hepatocytes surrounding the central vein, although mRNA expression levels were still low. At 6 h after PHx, a marked number of hepatocytes expressing mLECT2 protein and mRNA were seen throughout the liver, and at 12 h after PHx, hepatocytes expressing mLECT2 protein and mRNA further increased in number. However, expression levels of mLECT2 protein and mRNA at 24 It after PHx were significantly lower when compared with levels after 12 h. These results indicate that LECT2 triggers the early events of regeneration with concomitant suppression of hepatocyte proliferation.