RAPID STIMULATION OF AN OXIDATIVE BURST DURING ELICITATION OF CULTURED PLANT-CELLS - ROLE IN DEFENSE AND SIGNAL TRANSDUCTION
RAPID STIMULATION OF AN OXIDATIVE BURST DURING ELICITATION OF CULTURED PLANT-CELLS - ROLE IN DEFENSE AND SIGNAL TRANSDUCTION
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DOI：
10.1104/pp.90.1.109
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发表时间：
1989-05-01
期刊：
影响因子：
7.4
通讯作者：
LOW, PS
中科院分区：
文献类型：
--
作者：
APOSTOL, I;HEINSTEIN, PF;LOW, PS
用防御反应的激发子[来自大丽轮枝菌]刺激培养的植物细胞[Glycine max]，导致包括吲哚乙酸和某些荧光染料在内的各种水溶性化合物的快速破坏。这种破坏性的活动，这往往是积极表现在5分钟内的激发子添加，显示来自H2O2的快速生产和其使用的细胞外过氧化物酶。由于其出现的速度，这种氧化爆发可能有资格作为抵御入侵病原体的第一道诱导防线。由于H_2O_2在某些动物细胞中被认为是受植物激素刺激的代谢变化的第二信使，因此也研究了它在植物防御信号转导中的可能作用。不仅没有单独的外源H2O2刺激植物细胞悬浮液中的植保素生产，但抑制诱导剂刺激的植保素生产后，观察到添加过氧化氢酶和其他抑制剂的氧化爆发。此外，为了发生抑制，在诱导子添加过程中需要过氧化氢酶的存在，因为如果酶的引入延迟到添加诱导子后1小时，则不产生抑制。这些结果表明，H2O2也发挥了重要作用，在诱导随后的防御反应，如植物抗毒素的生产。
Stimulation of cultured plant cells [Glycine max] with elicitors of the defense response [from Verticillium dahliae] leads to the rapid destruction of a variety of water-soluble compounds including indoleacetic acid and certain fluorescent dyes. This destructive activity, which is often vigorously manifested within 5 minutes of elicitor addition, is shown to derive from the rapid production of H2O2 and its use by extracellular peroxidases. Because of its speed of appearance, this oxidative burst may qualify as the first induced line of defense against invading pathogens. Since H2O2 has been implicated as a second messenger of hormone-stimulated metabolic changes in some animal cells, its possible role in transduction of the defense signal in plants was also examined. Not only did exogenous H2O2 alone stimulate phytoalexin production in the plant cell suspension, but inhibition of elicitor-stimulated phytoalexin production was observed upon addition of catalase and other inhibitors of the oxidative burst. Furthermore, for inhibition to occur, the presence of catalase was required during elicitor addition, since if introduction of the enzyme was delayed until 1 hour after addition of the elicitor, no inhibition resulted. These results suggest that H2O2 also plays an important role in inducing subsequent defense responses such as phytoalexin production.