Tomato lipoxygenase D involved in the biosynthesis of jasmonic acid and tolerance to abiotic and biotic stress in tomato
Tomato lipoxygenase D involved in the biosynthesis of jasmonic acid and tolerance to abiotic and biotic stress in tomato
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DOI：
10.1007/s11816-015-0341-z
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发表时间：
2015-01-01
影响因子：
2.4
通讯作者：
Chen, Guoping
中科院分区：
文献类型：
--
作者：
Hu, Tingzhang;Hu, Zongli;Chen, Guoping
TomloxD 是一种来自 Lycopersicon esculentum Mill 的脂氧合酶，具有脂氧合酶活性。受伤、病原体感染、茉莉酸和系统素可刺激 TomloxD 的表达。为了研究TomloxD的功能，生产了沉默tomloxD基因的转基因番茄植物。 TomloxD表达的抑制导致脂氧合酶活性水平和内源茉莉酸含量显着降低，这表明TomloxD可以催化α-亚麻酸产生(13S)-氢过氧十八碳三烯酸(13-HPOT)，并且13-HPOT进一步代谢合成茉莉酸。实时RT-PCR显示，防御基因LeHSP90、LePR1和LePR6在转化体中的表达也低于野生型番茄植株。抗性测定表明，转基因番茄植株中TomloxD的抑制降低了番茄的耐热性并增加了其对黄枝孢菌的敏感性。总的来说，这里提供的数据表明，TomloxD 作为十八烷防御信号通路的一个组成部分发挥作用，并参与内源茉莉酸的生成，进而调节植物防御基因的表达以及对高温和病原体攻击的抵抗力。
TomloxD is a lipoxygenase from Lycopersicon esculentum Mill, which has lipoxygenase activity. The expression of TomloxD can be stimulated by wounding, pathogen infection, jasmonate, and systemin. To investigate the function of TomloxD, transgenic tomato plants silencing tomloxD gene was produced. The suppression of TomloxD expression led to a marked reduction in the levels of lipoxygenase activity and endogenous jasmonic acid content, which suggested TomloxD can catalyze alpha-linolenic acids to produce (13S)-hydroperoxyoctadecatrienoic acid (13-HPOT), and the 13-HPOT is metabolized further to synthesize jasmonic acid. Real-time RT-PCR revealed that the expression of defense genes LeHSP90, LePR1 and LePR6 was also less in the transformants than in the wild-type tomato plant. Resistance assay showed that the suppression of TomloxD in transgenic tomato plants reduced thermotolerance of tomato and increased its susceptibility to Cladosporium fulvum. Collectively, the data presented here suggest that the TomloxD plays a role as a component of the octadecanoid defense-signaling pathway and involved in the generation of endogenous jasmonic acid, and in turn regulates the expression of plant defense genes and resistance to high temperature and pathogen attack.