Regulation of sucrase-isomaltase gene expression along the crypt-villus axis of rat small intestine.
Regulation of sucrase-isomaltase gene expression along the crypt-villus axis of rat small intestine.
复制标题
大鼠小肠隐窝-绒毛轴蔗糖酶-异麦芽糖酶基因表达的调节。
DOI：
10.1016/s0006-291x(05)80853-8
复制
发表时间：
1990
影响因子：
3.1
通讯作者：
Traber,PG
中科院分区：
文献类型：
--
作者：
Traber,PG
应用差异分离细胞和原位杂交技术研究了蔗糖酶-异麦芽糖酶mRNA在大鼠小肠隐窝-绒毛轴上的表达。克隆了部分大鼠蔗糖酶-异麦芽糖酶cDNA，其编码的蛋白质与兔和人cDNA编码的蛋白质具有88%的同源性。大鼠基因组DNA的Southern杂交分析表明，该cDNA杂交到一个单一的基因。从分离自绒毛和隐窝的肠上皮细胞亚群中提取RNA，进行北方印迹，结果显示该cDNA与主要存在于绒毛RNA中的6.5 kb条带杂交。从绒毛中部到绒毛尖端，蔗糖酶-异麦芽糖酶mRNA的表达量呈下降趋势。我们的结论是，蔗糖酶异麦芽糖酶的表达，肠上皮细胞出现肠隐窝调节主要在mRNA积累的水平，这很可能是一个结果，激活蔗糖酶异麦芽糖酶基因转录。
The expression of sucrase-isomaltase mRNA was investigated along the crypt-villus axis of rat small intestine using differentially isolated cells andin situhybridization. A partial rat sucrase-isomaltase cDNA was cloned which coded for a protein that was predicted to be 88% homologous to those encoded by the rabbit and human cDNAs. Southern blot analysis of rat genomic DNA indicated that the cDNA hybridized to a single gene. Northern blots of RNA extracted from subpopulations of intestinal epithelial cells that were isolated from villus and crypt compartments showed that this cDNA hybridized to a 6.5 kb band predominantly in villus RNA.In situhybridization using35[S]-labeled RNA probes demonstrated that autoradiographic grains were detected over eptithelial cells located on villi with the greatest number of grains located at the crypt-villus junction and in the lower to mid-villus region; from mid-villus to the villus tip there was a decline in sucrase-isomaltase mRNA. We conclude that expression of sucrase-isomaltase as enterocytes emerge from intestinal crypts is regulated primarily at the level of mRNA accumulation which, most likely, is a result of activation of sucrase-isomaltase gene transcription.