Indirect immunoperoxidase test for the diagnosis of paratuberculosis.

Indirect immunoperoxidase test for the diagnosis of paratuberculosis.
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间接免疫过氧化物酶试验用于诊断副结核病。

DOI:
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发表时间:
1983
期刊:
影响因子:
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通讯作者:
Buergelt Cd
Buergelt Cd
中科院分区:
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文献类型:
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作者:
Ng Ht;Buergelt Cd

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本文报道了一种诊断副结核的间接免疫过氧化物酶方法。以福尔马林固定的含有大量副结核分枝杆菌的回盲部组织作为抗原源。以过氧化物酶标记的羊抗牛免疫球蛋白G为偶联物。该方法相对简单,可能被证明是一种常规的筛查试验。
: An indirect immunoperoxidase procedure for the diagnosis of paratuberculosis was described. Formalin-fixed ileocecal tissue containing large numbers of Mycobacterium paratuberculosis organisms was used as the source of antigen. Goat anti-bovine immunoglobulin G labeled with peroxidase was used as the conjugate in the test system. The method is relatively simple to do and may prove to be valuable as a routine screening test.