An antibody to de-N-acetyl sialic acid containing-polysialic acid identifies an intracellular antigen and induces apoptosis in human cancer cell lines.
An antibody to de-N-acetyl sialic acid containing-polysialic acid identifies an intracellular antigen and induces apoptosis in human cancer cell lines.
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DOI：
10.1371/journal.pone.0027249
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发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Moe GR
中科院分区：
文献类型：
--
作者：
Steirer LM;Moe GR
Polysialic acid (PSA), an α2,8-linked homopolymer of N-acetylneuraminic acid (Neu5Ac), is developmentally regulated and its expression is thought to be restricted to a few tissues in adults. Recently, we showed that two human pathogens expressed a derivative of PSA containing de-N-acetyl sialic acid residues (NeuPSA). Here we show that an epitope identified by the anti-NeuPSA monoclonal antibody, SEAM 3 (SEAM 3-reactive antigen or S3RA), is expressed in human melanomas, and also intracellularly in a human melanoma cell line (SK-MEL-28), a human T cell leukemia cell line (Jurkat), and two neuroblastoma cell lines (CHP-134 and SH-SY5Y). SEAM 3 binding induced apoptosis in the four cell lines tested. The unusual intracellular distribution of S3RA was similar to that described for the PSA polysialyltransferases, STX and PST, which are also expressed in the four cell lines used here. Interestingly, suppression of PST mRNA expression by transfection of SK-MEL-28 cells with PST-specific short interfering RNA (siRNA) resulted in decreased SEAM 3 binding. The results suggest further studies of the utility of antibodies such as SEAM 3 as therapeutic agents for certain malignancies.
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DOI：
10.1111/j.1550-7408.2010.00496.x
发表时间：
2010-09
期刊：
The Journal of eukaryotic microbiology
影响因子：
--
作者：
Iovannisci DM;Plested CP;Moe GR
通讯作者：
Moe GR
影响因子：
5.5
作者：
Flitter, Becca A.;Ing, Jessica Y.;Moe, Gregory R.
通讯作者：
Moe, Gregory R.
DOI：
10.1073/pnas.0905188106
发表时间：
2009-07-21
影响因子：
11.1
作者：
Drake, Penelope M.;Stock, Christina M.;Bertozzi, Carolyn R.
通讯作者：
Bertozzi, Carolyn R.
影响因子：
3.6
作者：
Moe, GR;Dave, A;Granoff, DM
通讯作者：
Granoff, DM
DOI：
10.1073/pnas.0912103107
发表时间：
2010-06-01
影响因子：
11.1
作者：
Galuska, Sebastian P.;Rollenhagen, Manuela;Geyer, Hildegard
通讯作者：
Geyer, Hildegard