Analysis of neuropeptides by perfusion liquid chromatography/electrospray ion-trap mass spectrometry.
Analysis of neuropeptides by perfusion liquid chromatography/electrospray ion-trap mass spectrometry.
复制标题
通过灌注液相色谱/电喷雾离子阱质谱分析神经肽。
DOI：
10.1002/rcm.1290080411
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发表时间：
1994
期刊：
影响因子：
--
通讯作者：
Voyksner,RD
中科院分区：
文献类型：
--
作者：
Lin,HY;Voyksner,RD
采用灌注高效液相色谱法（HPLC）联合电喷雾离子阱质谱法（ITMS）测定血浆中神经肽。灌注高效液相色谱提供了在5分钟内将神经肽分离到血浆中的能力，而在填充毛细管c18柱上进行分离需要30-60分钟。电喷雾结合ITMS提供了电离这些神经肽的能力，并以高灵敏度和特异性大量分析它们。在柱上注射的神经肽的亚皮摩尔量可以在血浆基质中特异性检测。每个神经肽的电喷雾- ITMS质谱显示出多个带电离子，可以用来确定或确认它们的分子量。
Perfusion high‐performance liquid chromatography (HPLC) combined with electrospray ion trap mass spectrometry (ITMS) was evaluated for the determination of neuropepetides in plasma. Perfusion HPLC offers the capability of resolving neuropeptides spiked into plasma in 5 min compared to the 30–60 min separations performed on packed capillary C18columns. Electrospray combined with the ITMS provides the ability to ionize these neuropeptides and mass analyze them with high sensitivity and specificity. Sub‐picomole quantities of neuropeptides injected on‐column could be specifically detected in a plasma matrix. The electrospray‐ITMS mass spectrum of each neuropeptide showed multiply charged ions which could be used to determine or confirm their molecular weights.