Accumulation of immature B and null lymphocytes in the periphery after intraperitoneal administration of traditional Chinese medicine, xiao-chai-hu-tang (Japanese name: shosaiko-to).
Accumulation of immature B and null lymphocytes in the periphery after intraperitoneal administration of traditional Chinese medicine, xiao-chai-hu-tang (Japanese name: shosaiko-to).
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腹腔注射中药小柴胡汤（日文名：正斋堂）后，未成熟B淋巴细胞和无效淋巴细胞在外周积聚。
DOI：
10.3109/08923978609026506
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
K. Nomoto
中科院分区：
文献类型：
--
作者：
T. Kawakita;A. Yamada;Y. Kumazawa;K. Nomoto
研究了腹腔注射小柴胡汤（日本名：shosaiko-to）后淋巴细胞的积累情况。Shosaiko-to在ICR小鼠腹腔内诱导淋巴细胞而非巨噬细胞明显聚集，而蛋白酶-蛋白胨、大肠杆菌脂多糖（LPS）、OK-432和细小棒状杆菌等多种刺激物诱导巨噬细胞优先聚集而非淋巴细胞。通过双色荧光活化细胞分选仪（FACS）分析发现，注射shosaio -to后，C3H/He小鼠腹腔和脾脏淋巴细胞亚群均增加，包括未成熟B细胞（IgM+和IgD-）和null细胞（thyl-和Ig-）。在其他C5正常株、C3H/HeJ （LPS-nonresponder）、C57BL/6、BALB/c和athymmic nu/nu （ICR背景）小鼠中均可见到shosaiko-to的这种作用，而在C5缺陷株、AKR/J、A/J和DBA/2小鼠中未见，说明shosaiko-to诱导外周未成熟B细胞和空细胞的积累与补体系统密切相关。
Accumulation of lymphocytes after an intraperitoneal (ip) injection of a traditional Chinese herb medicine, XIAO-CHAI-HU-TANG (Japanese name: shosaiko-to), was investigated. Shosaiko-to induced marked accumulation of lymphocytes rather than macrophages in the peritoneal cavity of ICR mice, whereas various kinds of irritants, e.g. proteose-pepton, Escherichia coli lipopolysaccharide (LPS), OK-432 and Corynebacterium parvum, induced preferential accumulation of macrophages rather than lymphocytes. By means of analysis using two-color fluorescence-activated cell sorter (FACS), it was revealed that the increased lymphocyte subpopulations not only in the peritoneal cavity but also in the spleen of C3H/He mice by the injection of shosaiko-to were comprised of both immature B (IgM+ and IgD-) and null (thyl- and Ig-) cells. This effect of shosaiko-to was observed in other C5 normal strains, C3H/HeJ (LPS-nonresponder), C57BL/6, BALB/c and athymic nu/nu (ICR background) mice, but not in C5 deficient strains, AKR/J, A/J and DBA/2 mice, indicating that the accumulation of immature B and null cells in the periphery induced by shosaiko-to is closely related to the complement system.
基因决定的对李斯特菌病的抵抗力与炎症性中性粒细胞和巨噬细胞积累的增加有关，而炎症性中性粒细胞和巨噬细胞具有增强的杀李斯特菌活性。
影响因子：
6.4
作者：
Czuprynski,CJ;Canono,BP;Henson,PM;Campbell,PA
通讯作者：
Campbell,PA
荧光激活细胞分选：理论、实验优化以及在淋巴细胞生物学中的应用。
影响因子：
--
作者：
Parks,DR;Herzenberg,LA
通讯作者：
Herzenberg,LA