Evaluation of Autologous Protein Solution Injection for Treatment of Superficial Digital Flexor Tendonitis in an Equine Model.
Evaluation of Autologous Protein Solution Injection for Treatment of Superficial Digital Flexor Tendonitis in an Equine Model.
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DOI：
10.3389/fvets.2021.697551
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发表时间：
2021
影响因子：
3.2
通讯作者：
Ortved KF
中科院分区：
文献类型：
--
作者：
Gaesser AM;Underwood C;Linardi RL;Even KM;Reef VB;Shetye SS;Mauck RL;King WJ;Engiles JB;Ortved KF
自体蛋白溶液（APS）已被传闻用于肌腱和韧带损伤的病灶内治疗，然而，其在这些损伤中的应用从未在体内进行过研究。我们的目的是评估 APS 对马浅屈肌 (SDF) 肌腱炎模型中肌腱愈合的影响。我们假设病灶内注射 APS 将导致更好的结构和生物力学愈合。使用胶原酶注射在八匹马的两个前肢中诱导 SDF 肌腱炎。一只前肢被随机分配接受 APS 病灶内注射，而另一只前肢注射生理盐水。在治疗后第-1、0、2、4、8和12周进行超声检查。第 12 周时，对马实施安乐死并采集 SDF 样本。进行了组织学评估、生物力学测试、基因表达分析、总糖胺聚糖 (GAG) 和总 DNA 定量。经盐水处理的肌腱中 III 型胶原蛋白 (COL3A1) 的表达显着高于正常肌腱 (p = 0.028)。除此之外，基因表达没有显着差异。各组之间的组织学或超声检查评分没有显着差异。盐水处理的肌腱中的平均总 DNA 含量显着高于正常肌腱 (p = 0.024)，而 APS 处理的肌腱和正常肌腱之间的总 DNA 含量没有显着差异。 APS 处理的肌腱的弹性模量高于盐水处理的肌腱，但差异并不显着。 APS 治疗肌腱中 COL3A1 表达的减少可能表明愈合良好。盐水处理的肌腱中总 DNA 含量的增加可能表明愈合过程正在进行，而 APS 处理的肌腱可能处于愈合的后期阶段。局限性包括研究周期相对较短以及诱发病变的大小和严重程度不一致。病灶内注射 APS 可以改善愈合特性。
Autologous protein solution (APS) has been used anecdotally for intralesional treatment of tendon and ligament injuries, however, its use in these injuries has never been studied in vivo. Our objective was to evaluate the effect of APS on tendon healing in an equine superficial digital flexor (SDF) tendonitis model. We hypothesized intralesional injection of APS would result in superior structural and biomechanical healing. SDF tendonitis was induced in both forelimbs of eight horses using collagenase injection. One forelimb was randomly assigned to receive an intralesional injection of APS, while the other was injected with saline. Ultrasonographic examinations were performed at weeks −1, 0, 2, 4, 8, and 12 following treatment. At 12 weeks, horses were euthanized and SDF samples harvested. Histologic evaluation, biomechanical testing, gene expression analysis, total glycosaminoglycan (GAG) and total DNA quantification were performed. Collagen type III (COL3A1) expression was significantly higher (p = 0.028) in saline treated tendon than in normal tendon. Otherwise, there were no significant differences in gene expression. There were no significant differences in histologic or ultrasonographic scores between groups. Mean total DNA content was significantly higher (p = 0.024) in saline treated tendons than normal tendons, whereas total DNA content was not significantly different between APS treated tendon and normal tendon. Elastic modulus was higher in APS treated than saline treated tendon, but the difference was not significant. Reduced expression of COL3A1 in APS treated tendon may indicate superior healing. Increased total DNA content in saline treated tendon may indicate ongoing healing processes, vs. APS treated tendons which may be in the later stages of healing. Limitations include a relatively short study period and inconsistency in size and severity of induced lesions. Intralesional injection of APS resulted in some improvements in healing characteristics.
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影响因子：
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作者：
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