Cloning and biochemical characterization of ToFZY, a tomato gene encoding a flavin monooxygenase involved in a tryptophan-dependent auxin biosynthesis pathway
Cloning and biochemical characterization of ToFZY, a tomato gene encoding a flavin monooxygenase involved in a tryptophan-dependent auxin biosynthesis pathway
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DOI：
10.1007/s00344-007-9019-2
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发表时间：
2007-12-01
影响因子：
4.8
通讯作者：
Perez, Jose A.
中科院分区：
文献类型：
--
作者：
Exposito-Rodriguez, Marino;Borges, Andres A.;Perez, Jose A.
吲哚-3-乙酸（Indole-3-acetic acid，IAA）是植物生长发育的重要调节因子。已经提出了多种途径用于IAA生物合成，但是不同途径的生理作用或相关性仍然不清楚。最近，四个成员的拟南芥YUC基因家族已牵连在一个额外的要求IAA参与花器官和维管组织的形成。拟南芥中的功能丧失的yuc 1 yuc 4双突变体显示出与先前描述的矮牵牛中的功能丧失的fzy突变体（fzy）相似的表型。此外，已经证明YUC 1编码黄素单加氧酶（FMO），其催化依赖于色氨酸的生长素生物合成途径的限速步骤：色胺转化为N-羟基色胺。在这里，我们报告的遗传研究ToFZY，推定的番茄直系同源YUC 4和FZY，包括基因和cDNA序列比较和初步的表达分析。此外，我们描述了一种新的保守的氨基酸基序，可能被认为是一个标志，可能用于识别新的YUC样FMO。我们还证明了ToFZY编码的蛋白质具有与YUC 1相同的酶活性。最后，我们提供的证据表明，ToFZY基因属于一个多基因家族，其成员可能表现出类似于A. thaliana.
Indole-3-acetic acid (IAA), the main endogenous auxin, has been known for decades to be a key regulator for plant growth and development. Multiple routes have been proposed for IAA biosynthesis but physiologic roles or relevance of the different routes are still unclear. Recently, four members of the Arabidopsis thaliana YUC gene family have been implicated in an additional requirement of IAA involved in floral organ and vascular tissue formation. The loss-of-function yuc1yuc4 double mutants in Arabidopsis displayed phenotypes similar to the previously described loss-of-function floozy mutants in petunia (fzy). Moreover, it has been demonstrated that YUC1 encodes a flavin monooxygenase (FMO) that catalyzes a rate-limiting step of a tryptophan-dependent auxin biosynthesis pathway: the conversion of tryptamine to N-hydroxyl-tryptamine. Here we report on the genetic study of ToFZY, the putative tomato ortholog of YUC4 and FZY, including gene and cDNA sequence comparison and a preliminary expression analysis. In addition, we describe a novel conserved amino acid motif that may be considered a hallmark potentially useful for the identification of new YUC-like FMOs. We also demonstrate that ToFZY encodes a protein with the same enzymatic activity as YUC1. Finally, we provide evidence suggesting that the ToFZY gene belongs to a multigenic family whose members may exhibit a temporal and spatial specialization similar to that described in A. thaliana.