Aptamer-based folding fluorescent sensor for cocaine
Aptamer-based folding fluorescent sensor for cocaine
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DOI：
10.1021/ja0038171
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发表时间：
2001-05-30
影响因子：
15
通讯作者：
Landry, DW
中科院分区：
文献类型：
--
作者：
Stojanovic, MN;de Prada, P;Landry, DW
我们在两个步骤中调整了基于脱氧核糖核苷酸的适体以发出可卡因识别的信号：在形成可卡因结合口袋的三向连接的一个茎中设计了不稳定性，并且用荧光团和猝灭剂标记了所得的短茎。在没有可卡因的情况下，两个茎是开放的，但在可卡因的存在下，它们关闭并形成三通连接。这种主要的结构变化将荧光团和猝灭剂结合在一起，从而发出配体存在和浓度的信号。该传感器对可卡因的选择性超过其代谢物。可在血清中操作，并可用于可卡因水解酶的筛选。
We adapted in two steps a deoxyribonucleotide-based aptamer to signal the recognition of cocaine: an instability was engineered in one stem of a three-way junction that forms the cocaine-binding pocket and the resulting short stern was end labeled with a fluorophore and a quencher. In the absence of cocaine, two stems are open, but in its presence they close and the three-way junction forms. This major structural change brings fluorophore and quencher together thereby signaling the presence and concentration of ligand. The sensor is selective for cocaine over its metabolites. can operate in serum, and is useful for the screening of cocaine hydrolases.