vir90, a virulence-activated gene coding for a Bordetella pertussis iron-regulated outer membrane protein
vir90, a virulence-activated gene coding for a Bordetella pertussis iron-regulated outer membrane protein
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DOI：
10.1016/s0923-2508(03)00115-3
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发表时间：
2003-07-01
影响因子：
2.6
通讯作者：
Franco, MA
中科院分区：
文献类型：
--
作者：
de Rossi, BNP;Friedman, LE;Franco, MA
百日咳杆菌受bvgAS基因座调控的表型变化，调控着许多与毒力和免疫原性相关的基因的表达。我们先前报道了百日咳杆菌90 kDa BVG调控的外膜蛋白(OMP)的N端序列(Swiss-Prot登录号：P81549)，我们将其命名为Vir90。通过对百日咳杆菌、副百日咳杆菌和败血杆菌基因组序列的计算机分析，确定了百日咳杆菌、副百日咳杆菌和败血杆菌中可能编码病毒90的开放阅读框(ORF)。对Vir90上游区的核苷酸序列分析表明，存在一个可能的启动子、一个BvgA结合位点和一个可能的Fur结合位点。百日咳杆菌Vir90蛋白与革兰氏阴性细菌的铁载体受体有显著的同源性。抗Vir90His重组蛋白的抗血清可识别这三种强毒波尔德氏菌OMP免疫印迹中的90 kDa蛋白。在低铁生长条件下，Vir90蛋白的积累增加了4倍。因此，Vir90基因在受试物种中表达，其表达受BvgAS系统的正调控，在高铁浓度下表达负调控，可能受Fur调控。(C)2003年版《爱思唯尔科学与医学》。版权所有。
Bordetella pertussis undergoes phenotypic changes modulated by the bvgAS locus, which regulates the expression of many genes related to virulence and immunogenicity. We previously reported the N-terminal sequence of a 90 kDa bvg-regulated outer membrane protein (OMP) of B. pertussis (SWISS-PROT accession No. p81549), a novel potential virulence factor that we named Vir90. The open reading frames (ORFs) which potentially code for Vir90 in B. pertussis, B. parapertussis and B. bronchiseptica were identified by computer analysis of the genomic sequences available for the three Bordetella species. Nucleotide sequence analysis of the vir90 upstream region revealed the presence of a putative promoter, a BvgA binding site and a putative Fur binding site. The B. pertussis Vir90 protein showed significant homology with ferrisiderophore receptors from Gram-negative bacteria. An antiserum raised against Vir90His recombinant protein recognized the 90-kDa protein in immunoblots of OMPs from these three virulent Bordetella species. The accumulation of the Vir90 protein increased 4-fold under low iron growth conditions. Therefore, the vir90 gene is expressed in the tested species and its expression is regulated positively by the BvgAS system and negatively under high iron concentration, likely by Fur. (C) 2003 Editions scientifiques et medicales Elsevier SAS. All rights reserved.