Functional characterization of optimized acellular peripheral nerve graft in a rat sciatic nerve injury model
Functional characterization of optimized acellular peripheral nerve graft in a rat sciatic nerve injury model
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DOI：
10.1179/1743132810y.0000000023
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发表时间：
2011-07-01
影响因子：
1.9
通讯作者：
Schmidt, Christine E.
中科院分区：
文献类型：
--
作者：
Nagao, Ryan J.;Lundy, Scott;Schmidt, Christine E.
目的：脱细胞移植物是神经重建手术的一种可行选择。最近，我们的实验室创造了一种新的优化脱细胞程序，去除免疫物质，同时保留大部分细胞外基质结构完整。优化的脱细胞（OA）移植物已被证明引起的免疫反应等于或小于引起的同种异体移植物，在大鼠模型中的自体移植物的类似物。我们研究了OA移植物的性能，以提供功能恢复在长期study.Methods：我们进行了长期的功能再生评估研究，使用坐骨神经功能指数，以量化恢复刘易斯大鼠在定期的时间间隔长达52周移植植入后1厘米坐骨神经切除术。OA移植物进行了比较，对其他脱细胞的方法（Sondell治疗和热脱细胞），以及isograft和原发neurrhaphy.Results：OA移植支持可比的功能恢复的isograft和上级再生热和Sondell脱细胞方法。此外，OA移植促进早期恢复到更大的程度相比，使用热或Sondell方法获得的脱细胞移植物。讨论：同等功能恢复的isograft表明，OA神经移植可能是未来的临床替代目前的自体组织移植。
Objectives: Acellular grafts are a viable option for use in nerve reconstruction surgeries. Recently, our lab created a novel optimized decellularization procedure that removes immunological material while leaving the majority of the extracellular matrix structure intact. The optimized acellular (OA) graft has been shown to elicit an immune response equal to or less than that elicited by the isograft, the analog of the autograft in the rat model. We investigated the performance of the OA graft to provide functional recovery in a long-term study.Methods: We performed a long-term functional regeneration evaluation study using the sciatic functional index to quantify recovery of Lewis rats at regular time intervals for up to 52 weeks after graft implantation following 1 cm sciatic nerve resection. OA grafts were compared against other decellularized methods (Sondell treatment and thermal decellularization), as well as the isograft and primary neurorrhaphy.Results: The OA graft supported comparable functional recovery to the isograft and superior regeneration to thermal and Sondell decellularization methods. Furthermore, the OA graft promoted early recovery to a greater degree compared to acellular grafts obtained using either the thermal or the Sondell methods.Discussion: Equivalent functional recovery to the isograft suggests that the OA nerve graft may be a future clinical alternative to the current autologous tissue graft.