A Novel Mediation Strategy of DSS-Induced Colitis in Mice Based on an Iron-Enriched Probiotic and In Vivo Bioluminescence Tracing.
A Novel Mediation Strategy of DSS-Induced Colitis in Mice Based on an Iron-Enriched Probiotic and In Vivo Bioluminescence Tracing.
复制标题
DOI：
10.1021/acs.jafc.0c05260
复制
发表时间：
2020-10
影响因子：
6.1
通讯作者：
Ning Zhao;Jingmin Liu;Feier Yang;Xuemeng Ji;Chun-Yang Li;Shi-Wen Lv;Shuo Wang
中科院分区：
文献类型：
--
作者：
Ning Zhao;Jingmin Liu;Feier Yang;Xuemeng Ji;Chun-Yang Li;Shi-Wen Lv;Shuo Wang
由失血和/或铁吸收减少引起的铁缺乏（ID）是影响炎症性肠病（IBD）健康的严重问题。然而，传统的铁补充剂可能无法满足IBD的无副作用需求；因此，迫切需要开发一种新的铁补充剂。本研究首次从中国传统发酵食品中筛选具有富铁能力的益生菌食物乳杆菌NKU556，并用生物发光示踪法干预dss诱导的小鼠结肠炎。正如预期的那样，口服NKU556-Fe可以显著提高dss诱导的小鼠结肠炎紧密连接蛋白的表达，有效降低促炎细胞因子和氧化应激。同时，与FeSO4组相比，NKU556-Fe的摄入可抑制肝脏源性hepcidin的表达，减少FPN1的降解，从而导致结肠炎小鼠铁吸收增加。根据生物发光结果，我们认为口服NKU556/NKU556- fe对dss诱导小鼠结肠炎的有益作用与其代谢产物无关，而与其自身功能有关。上述结果表明，口服NKU556-Fe可减轻结肠炎炎症，增加铁的吸收。综上所述，目前的工作不仅为IBD提出了一种新的介导策略，也为未来肠外并发症的治疗提供了一些启示。
Iron deficiency (ID) caused by blood loss and/or reduced iron absorption is a serious problem influencing health in inflammatory bowel disease (IBD). However, traditional iron supplements may fail to meet no side effect demands for ID of IBD; thus, a new iron supplementation is highly desired to be developed. Herein, for the first time, probiotic Lactobacillus alimentarius NKU556 with an iron-enriching ability was screened from Chinese traditional fermented food then employed to intervene DSS-induced colitis with bioluminescence tracing in mice. As expected, oral administration with NKU556-Fe can remarkably enhance the expression of tight junction proteins and effectively reduce the pro-inflammatory cytokines as well as the oxidative stress on DSS-induced colitis in mice. Meanwhile, in comparison with the FeSO4 group, the intake of NKU556-Fe could suppress the expression of hepcidin derived from the liver and reduce the degradation of FPN1, thereby leading to the increase in the iron absorption of colitis in mice. According to the bioluminescence result, it was believed that the beneficial effects of oral administration with NKU556/NKU556-Fe on DSS-induced colitis in mice were hardly related to its metabolites but associated with its own function. These results concluded that the oral administration of NKU556-Fe could relieve colitis inflammation and increase iron absorption. In summary, current work not only proposed a novel mediation strategy for IBD but also offered some inspirations for future treatment of extraintestinal complications.