The occurrence of circulating immune complexes and viral antigens in idiopathic thrombocytopenic purpura.
The occurrence of circulating immune complexes and viral antigens in idiopathic thrombocytopenic purpura.
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发表时间：
1977-04
影响因子：
4.6
通讯作者：
A. Lurhuma;H. Riccomi;P. Masson
中科院分区：
文献类型：
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作者：
A. Lurhuma;H. Riccomi;P. Masson
用RF或C1 q法检测了72例ITP患者血清对IgG包被颗粒凝集的抑制活性。大多数（83%）显示对两种凝集剂的抑制作用，而17%被发现含有内源性RF。血小板数与抑制因子滴度呈负相关。一些血清通过凝胶过滤分级。抑制因子和有时痕量的IgG分布在单体7S IgG之前洗脱的几个峰上。在两个ITP血清的重馏分中检测到的IgG对应于抗原-抗体复合物所示的解离实验在酸性pH值。在所有ITP血清色谱分析，DNA已被检测到的重馏分，似乎是某些复合物的抗原。用反向电泳法分析了42例ITP患者血清中病毒抗原的存在。20份血清中检测到HBs抗原，5份血清中检测到EBV抗原，6份血清中检测到腺病毒抗原。
The sera of seventy-two patients with ITP were tested for their inhibitory activity on the agglutination of IgG-coated particles by RF or C1q. The majority (83%) displayed an inhibitory effect toward both agglutinators, whereas 17% were found to contain endogenous RF. A negative correlation was observed between the number of platelets and the titres of inhibitory factors. Some sera were fractionated by gel filtration. The inhibitory factors and sometimes trace amounts of IgG were distributed over several peaks eluted before monomeric 7S IgG. The IgG detected in the heavy fractions of two ITP sera corresponded to antigen-antibody complexes as shown by dissociation experiments at acid pH. In all ITP sera analysed by chromatography, DNA has been detected in the heavy fractions and appears to be the antigen of certain complexes. The sera from forty-two patients with ITP were analysed by counter-electrophoresis for the presence of viral antigens. HBs antigen was detected in twenty sera, EBV antigen in five, and adenovirus antigen in six.