Histopathologic and immunohistochemical comparison of human, rabbit, and swine aneurysms embolized with platinum coils.
Histopathologic and immunohistochemical comparison of human, rabbit, and swine aneurysms embolized with platinum coils.
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发表时间：
2005-11
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通讯作者：
D. Dai;Y. Ding;M. A. Danielson;R. Kadirvel;D. Lewis;H. Cloft;D. Kallmes
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作者：
D. Dai;Y. Ding;M. A. Danielson;R. Kadirvel;D. Lewis;H. Cloft;D. Kallmes
背景与目的本研究的目的是通过比较兔和猪实验性动脉瘤与铂圈栓塞人动脉瘤的组织学和免疫组织化学结果，阐明动脉瘤愈合的细胞机制。方法制备猪侧壁动脉瘤（n = 5, 12周取材）和兔弹性酶诱导动脉瘤（n = 6, 24周取材）并栓塞。在死前6年被栓塞的单个人动脉瘤，在尸检后被采集。所有标本均采用改良石蜡包埋技术处理。组织切片，苏木精、伊红、马松三色染色。免疫组织化学和免疫荧光检测多种抗体，包括α -平滑肌肌动蛋白、肌球蛋白重链、desmin、vimentin和CD31。结果人动脉瘤穹窿内充满疏松、细胞少、无定形组织。动脉瘤的颈部完全被一层薄薄的细胞下组织覆盖。穹顶和颈部胶原和肌成纤维细胞稀疏。兔动脉瘤的圆顶也充满了松散的、细胞少的、无定形的基质。在6个动脉瘤中的5个，一层薄薄的细胞下层组织沿着颈部延伸。穹窿中胶原和肌成纤维细胞稀疏。猪动脉瘤充满了密集浸润的组织，包括慢性炎症组织和与肌成纤维细胞相关的广泛的、减薄的胶原纤维束。厚层肌成纤维细胞完全桥接了颈部。结论：铂线圈栓塞在兔模型中见不到胶原沉积和肌成纤维细胞反应，而在猪模型中未见。兔弹性酶诱导的动脉瘤模型比侧壁猪动脉瘤更适合用于改善动脉瘤内纤维化的改良装置的试验。
BACKGROUND AND PURPOSE The purpose of this study was to clarify the cellular mechanisms of aneurysmal healing by comparing histologic and immunohistochemical findings in experimental rabbit and swine aneurysms to a human aneurysm embolized with platinum coils. METHODS Swine sidewall aneurysms (n = 5, harvested at 12 weeks) and elastase-induced rabbit aneurysms (n = 6, harvested at 24 weeks) were created and embolized. A single human aneurysm, embolized 6 years before death, was harvested following autopsy. All specimens were processed by using a modified paraffin embedding technique. Tissue was sectioned and stained with hematoxylin and eosin and Masson trichrome. Immunohistochemistry and immunofluorescence were performed with multiple antibodies, including alpha smooth muscle actin, myosin heavy chain, desmin, vimentin, and CD31. RESULTS The human aneurysm's dome was filled with loose, hypocellular, amorphous tissue. The aneurysm's neck was completely covered with a thin layer of hypocellular tissue. Collagen and myofibroblasts were sparse in both the dome and neck. Rabbit aneurysms' domes were also filled with a loose, hypocellular tissue, amorphous matrix. In 5 of 6 aneurysms, a thin layer of hypocellular tissue ran along the neck. Collagen and myofibroblasts were sparse in the dome. Swine aneurysms were filled with densely infiltrated tissue, including chronic inflammatory tissue and extensive, attenuated collagen fiber bundles associated with myofibroblasts. Thick layers of myofibroblasts entirely bridged the necks. CONCLUSIONS Absence of collagen deposition and scant myofibroblastic reaction to platinum coil embolization are seen in the rabbit model but not in swine aneurysms. The elastase-induced aneurysm model in rabbits is more suitable than sidewall swine aneurysms for testing of modified devices aimed at improving intra-aneurysmal fibrosis.