Production of Right Ventricular Hypertrophy With and Without Congestive Heart Failure in the Gat
Production of Right Ventricular Hypertrophy With and Without Congestive Heart Failure in the Gat
复制标题
伴有或不伴有充血性心力衰竭的右心室肥大的产生
DOI：
10.3181/00379727-125-32137
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发表时间：
1967
影响因子：
--
通讯作者：
E. Sonnenblick
中科院分区：
文献类型：
--
作者：
J. Spann;R. Buccino;E. Sonnenblick
已经在狗(L)、豚鼠(2)和兔(3)中诱导了心室肥厚和充血性心力衰竭，这些实验模型提供了对这些病理状态的相当深入的了解。然而，从肥厚或衰竭的心脏分离出来的心肌收缩状态的定量描述是不可用的。CAT在这些情况下为分析心肌的收缩状态提供了独特的优势，因为来自右心室的乳头肌足够小，可以进行体外充氧，其纤维平行定向，从而可以定义单位肌肉横截面积和长度的心肌功能。此外，猫乳头状肌制剂的功能特征已被详细描述(4)，并且足够均匀，以允许有意义地比较一组猫与另一组猫的心肌收缩状态(5)。为了给这类研究提供动物模型，已经建立了一种猫右室肥厚伴和不伴充血性心力衰竭的方法，这是本报告的主题。这是通过减少主肺动脉瓣膜上部分的管腔对右心室施加慢性压力负荷来实现的。方法：研究方法。用圆形夹子(6)(图1)收缩猫的肺动脉，其应用方式如下。动物静脉注射甲氧己醇钠(15 mg/kg)麻醉。为便于气管插管和控制呼吸，静脉注射琥珀胆碱(1 Mg Kg)，采用哈佛呼吸机∗空气间歇正压呼吸机。在无菌条件下，从距心尖冲动头2 cm的左侧肋间前1/3切开胸腔，广泛切除心包，分离升主动脉后，切除主肺动脉5 mm段，远离升主动脉。
Ventricular hyperthrophy and congestive heart failure have been induced in dogs(l), guinea pigs(2), and rabbits(3) and such experimental models have provided considerable insight into these pathologic states. However, a quantitative description of the contractile state of cardiac muscle isolated from the hypertrophied or failing heart is not available. The cat offers unique advantages for analyzing the contractile state of the myocardium in these conditions because the papillary muscle from the right ventricle is small enough to permit in vitro oxygenation and its fibers are oriented in parallel and thus allow definition of heart muscle function per unit of muscle cross-sectional area and length. Further, the functional characteristics of the cat papillary muscle preparation have been described in detail(4) and are sufficiently uniform to allow meaningful comparison of the myocardial contractile state of one group of cats to that of another group(5). To provide the animal model for such studies, a method for production of right ventricular hypertrophy with and without congestive heart failure in the cat has been developed and is the subject of this report. This was accomplished by imposing a chronic pressure load on the right ventricle by reduction of the lumen of the supravalvular portion of the main pulmonary artery. Methods. The pulmonary artery of the cats was constricted by a circular clip(6) (Fig. 1) which was applied in the following manner. Animals were anesthetized with intravenous sodium methohexital (15 mg/kg). To permit endotracheal intubation and control of respiration intravenous succinylcholine (1 mg kg) was then given and intermittent positive pressure ventilation was applied by a Harvard respirator∗ using air. Under sterile conditions, the chest was opened through the anterior one-third of that left intercostal space lying 2 cm cephalad from the apical impulse, the pericardium was widely excised, and a 5 mm segment of the main pulmonary artery just distal to the pulmonic valve was dissected free of the ascending aorta.